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RNase Inhibitor, recombinant, GMP Grade

from rat lung, expressed in E. coli, solution

For further processing only.

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RNase Inhibitor, recombinant, GMP Grade material number and pack size:
Material Number Pack Size
04488105103 100 kU
03247058103 1.4 MU
04488105103: Will be supplied as "RNase Inhibitor Ind. GMP Grade, 100 KU". Unit of measure is "piece".
03247058103: Will be supplied as "RNAse Inh. GMP, Blk 1.4M U/bottle". Unit of measure is "MU".
RNase Inhibitor, recombinant, GMP Grade, is useful in any application where protection from RNA degradation by RNases is critical.
These are provided in polypropylene (PP) bottles; 04488105103 is supplied in a 8 ml Nalgene PP bottle.
Use RNase Inhibitor, recombinant, GMP Grade, to protect RNA from unwanted degradation in diagnostic and therapeutic applications:
  • in vitro transcription to generate mRNA therapeutics,
  • cDNA synthesis reactions,
  • protection of RNA during RNA isolation.
RNase Inhibitor, recombinant, GMP Grade, is a protein of 50kD that inhibits enzymatic activity of RNases by noncovalently binding to the active site.
Activity: A minimum of 1 mmol/L DTT is required to maintain the inhibitor in its active state; a pH value between 5.0 and 9.0 is recommended (isoelectric point is at pH 4.7).
Inactivation: At temperatures >+65°C or under severe denaturing conditions the inhibitory activity disappears.
Appearance: Clear, colorless solution
Volume activity (with cCMP/RNAse A): > 40 x 103 U/mL
Specific activity: ≥80 kU/mg protein
Storage buffer: Hepes-KOH, 20 mmol/L; KCl, 50 mmol/L; Glycerol, 50% (v/v); DTT, 5 mmol/L; pH approximately 7.6
Unit definition: One unit RNase Inhibitor, recombinant, GMP Grade, is defined as the amount that inhibits 50% of the activity of 5 ng RNase A (activity measured according to Blackburn by inhibition of hydrolysis of cyclic cytidine-monophosphoric acid).
Purity (SDS PAGE): >95%
Bioburden: <50 CFU/mL
DNA content: <100 pg/mg protein
Unspecific nucleases (up to 400 U using MWM III-DNA/1 h/37°C): Not detectable.
Nicking activity (up to 400 U using pBR 322-DNA/1 h/37°C): Not detectable.
RNase activity (up to 400 U using MS 2-RNA/1 h/37°C): Not detectable
RNase activity after thermal inactivation (10 min/65°C): Not detectable up to 160 U using MS 2-RNA/1 h/37°C
Performance test on Titan-kit (Ident-no: 11 939 823): Corresponds to specification
Performance test on LightCycler® (PBGD system): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.