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Lyo-ready enzymes and reagents

Lyo-ready: glycerol-free and high concentration reagents for point of care and other molecular assays


Roche CustomBiotech lyo-ready reagents are a unique portfolio of high-performance enzymes that streamline the development and production of assays intended for molecular PoC diagnostics.

The highly concentrated and glycerol-free PCR reagents can be dried down directly. As a result, they can be employed in a wide range of assay formats, allowing more flexibility for development and cost saving during commercialization.

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Gains from working with lyo-ready reagents

Glycerol-free formulation = no glycerol removal:

  • can cut reagent loss by up to 20%

  • saves additional processing steps

  • no testing post-glycerol removal necessary

High concentration = lower reagent volumes

  • saves volume for more sample material

  • boosts sensitivity

  • fewer limitations on development strategies

Lyo-ready reagents couple high performance and low cost for PoC and other molecular assays.

Our lyo-ready enzymes enable fast-cycling protocols, and are tolerant to inhibitors. These features simplify assay handling and minimize the number and amount of reagents needed for protocol steps, from sample preparation to detection reactions. That efficiency translates to lower costs per reaction, particularly important in the point-of-care setting.


Discover how the right reagents reduce costs in mPOC with our infographic

kapa 3g amplification curves

Figure 1. Lyophilized glycerol-free KAPA3G HotStart DNA polymerase retains enzymatic activiy even at high storage temperatures. 
Stored at 37°C for over 4 months, the lyohilized format delivers the same high performance as non-lyophilized enzyme stored at -20°C.

Data on file at Roche.

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Regulatory disclaimer are listed on respective product pages.

Find the optimal solution for the lyophilization of your molecular diagnostics assay and choose from our lyo-ready and high concentration reagents:

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