NucleoMix (dUTP), PCR Grade
sodium salt, 100 mM (25 mmol/L each dNTP)
For further processing only.
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Will be supplied as "NucleoMix PCR Grd (dU), 25 mmol/L, 20 mL". Unit of measure is "mL".
NucleoMix (dUTP), PCR Grade material number and pack size: Material Number Pack Size 04980905103 20 mL - GMP-manufactured NucleoMixes from the leading manufacturer of nucleotides for outstanding, consistent performance in amplification reactions. NucleoMix (dUTP), PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
- NucleoMix (dUTP), PCR Grade, is designed for amplification reactions where high-quality reagents are required, such as for in vitro diagnostics. The ready-to-use mix eliminates process steps. Avoid carryover contamination between PCRs, a significant source of false positives. To decontaminate PCR and RT-PCR reagent mixes, dUTP is incorporated in place of dTTP. Subsequent reactions should be treated with Uracil-DNA Glycosylase (UNG) before amplification to degrade dUTP-containing PCR products.
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Appearance: Clear, colorless solution
pH value: 8.1-8.5
Concentration (sum, 1 μmol ≙ 11.0 A260 units, pH 7.0): 100-110 mmol/L
Identity (HPLC diode array detector):
dATP: 23-28 mmol/L
dCTP: 23-28 mmol/L
dGTP: 23-28 mmol/L
dUTP: 23-28 mmol/L
dNTP (HPLC, sum of 4 peaks): ≥99 area%
dNDP (HPLC, sum of 4 peaks): ≤0.9 area%
DNases/RNases: Negative
Nicking activity: Negative
Function test in PCR (RNA, human dystrophin gene): Corresponds to specification
Stability: At -15 to -25°C within specification range for 30 months.