• Broad spectrum

    of products for

    your industry

Trypsin, recombinant

expressed in Pichia pastoris

For further processing only.

expand | collapse
Order information
Trypsin, recombinant material number and pack size:
Material Number Pack Size
03358658103 3.5 MU
06369880103 1 g = 0.23 MU
03 358 658 103: Will be supplied as "Trypsin rec. Bulk". Unit of measure is "MU".
06 369 880 103: Will be supplied as "Trypsin, recombinant 1g". Unit of measure is “piece”.
Trypsin, recombinant, is intended to use in highly regulated production processes at pharmaceutical companies.
Trypsin is a widely used serine protease, typically isolated from pancreas of different animals, that specifically cleaves at the C-terminus of arginine and lysine within a peptide chain. Roche has chemically synthesized a gene encoding for the amino acid sequence of Trypsin and has transformed the gene into the expression host Pichia pastoris, which expresses the recombinant Trypsin as active protease with identical properties compared to the native Trypsin.
The product is manufactured according to DIN EN ISO 13485. No animal-derived products are used in the fermentation, purification and final formulation. The production process is validated resulting in a very high lot-to-lot consistency.
  • Minimize the risk of virus contamination and the risk of animal-related cross-contamination.
  • Rely on high purity. Minimize the risk of host cell protein contamination in your final product.
  • Increase the safety of your production processes with reproducible performance and high lot-to-lot consistency.
Use the animal component-free and DIN EN ISO 13485-manufactured Trypsin, recombinant, as critical raw material for the production of active pharmaceutical ingredients (API), i.e., insulin, vaccines and for cell dissociation.
Molecular weight: 23.5 kD
pH optimum: 8.0
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain
Appearance: Clear, colorless to slightly yellowish solution
Storage buffer: HCl, 10 mmol/L; CaCl2, 20 mmol/L
pH value: 2±0.5
Activity (with chromozym TRY): ≥10,800 U/mL
Total activity (with chromozym TRY): 3.5 MU ±10%
Specific activity: ≥180 U/mg
Protein: 70±10 mg/mL
Purity (RP-HPLC): ≤20% α-trypsin, ≥70% β-trypsin, corresponds to master lot
Bioburden: ≤100 CFU/mL
Stability: At -15 to -25°C within specification range for 36 months.