Blended Proteolytic Enzyme for Tissue Dissociation. For Further Processing only. Others Collagenase I II GMP Grade Collagenase I/II, GMP Grade 3.5.3.3.13.1 lyophilizate, mammalian free, from Chlostridium histolyticum 05 349 907 103 5 349 907 103 05349907103 5349907103 05349907103 Collagenase I/II, GMP-Grade Collagenase I/II, GMP-Grade 07613336163412 Reagents, kits 1 bottle Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Collagenase I/II, mammalian free". Unit of measure is "piece". 9001-12-1 en Collagenase I/II, GMP Grade is a precision-blended enzymatic reagent containing purified collagenase Class I and Class II from Clostridium histolyticum. Formulated completely free of mammalian-derived raw materials, this lyophilized blend combines the complementary digestion dynamics of both isoforms into a defined, ready-to-reconstitute formulation. Designed for specialized tissue dissociation applications, Collagenase I/II provides high total enzymatic purity, low endotoxin levels, and batch-to-batch consistency required to maximize target cell yields and maintain high cell viability in commercial manufacturing and research workflows. en Appearance white lyophilized cake correspondsProtein mass / bottle (target: 537 mg protein) 430 - 644 mg proteinCollagenase I content (target 322 mg): Range/value 258 - 386 mgCollagenase II content (target 215 mg): Range/value 172 - 258 mgCollagenase I b,c part of Collagenase I: ≤10% (area)HPLC purity total peak areas (Col I + Col II): ≥85%Activity (Wünsch) calculated 2172 – 3617 U/vial Ratio Collagenase II / Total Collagenase (HPLC) 0.3– 0.5Endotoxins ≤ 10 EU/mg proteinBioburden: 10 CFU/bottle correspondsStability at -25°C to -15°C: within specification range for 47 months. en Highly purified, mammalian-free lyophilized enzyme blend delivering optimized Class I and Class II collagenase ratios for reproducible, high-yield tissue dissociation in regulated bioprocesses. en Standardized tissue dissociation: Enzymatic breakdown of complex extracellular matrices requiring a balanced combination of Class I and Class II collagenases.Primary cell isolation: High-yield single-cell isolation protocols for research and commercial bioprocessing where high cell recovery and viability are critical.Cell and gene therapy manufacturing: Standardized, high-purity tissue digestion step for regulated commercial cell therapy processes. en

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Collagenase I/II, GMP Grade

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