NxtScript 2G RT, conc.
thermostable reverse transcriptase, 500 U/μL, glycerol-free solution
For further processing only.
NxtScript 2G RT, conc. material number and pack size: Material Number Pack Size 09085220103 custom fill 500 U/μL
Highly concentrated, glycerol-free reverse transcriptase developed for high temperature reactions.
NxtScript 2G RT, conc. is a stand-alone reverse transcriptase. The highly concentrated and glycerol-free formulation makes the product suitable for lyophilization and for the preparation of dry amplification mixes.
Improve sensitivity and specificity
- high thermostability - no loss of activity even at the reaction temperature higher than +65°C for improved handling of secondary structures and difficult targets.
Easily lyophilize your assays
-high concentration (500 U/uL) and glycerol-free formulation allowing easy lyophilization and drying down.
- Make use of the wide temperature activity range of NxtScript 2G RT and reverse transcribe at the temperature that is optimal for your RNA target.
- Use NxtScript 2G RT for synthesis of cDNA from total RNA or mRNA for:
Two-step and one-step RT-PCR applications
RT-PCR for detection of viral targets
RT-PCR for detection of mRNA targets, such as cancer biomarkers.
NxtScript 2G reverse transcriptase is highly thermostable and allows high temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Recommended reaction temperature: +55 to +70°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg2+ Appearance: Clear, colourless solution
Volume activity: > 500 U/μL
Nicking activity (pBR 322 DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
RNases (MS2 RNA): Not detectable in up to 75 U after 1 hour incubation at +37°C.
E. coli DNA: ≤ 100 pg E.coli DNA/mL enzyme
Human DNA: ≤ 15 ng human DNA/mL enzyme
Performance test: TwoStep RT-qPCR, β-Actin (on LC480 II with human reference RNA and β-Actin assay)
Stability: At -15 to -25°C within specification range for 12 months.