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NxtScript 2G RT, conc.

thermostable reverse transcriptase, 500 U/μL, glycerol-free solution

For further processing only.

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Order information
NxtScript 2G RT, conc. material number and pack size:
Material Number Pack Size
09085220103 custom fill 500 U/μL
Will be supplied as "NxtScript 2G RT, conc.". Unit of Measure is "kU".
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Overview
Highly concentrated, glycerol-free reverse transcriptase developed for high temperature reactions.
NxtScript 2G RT, conc. is a stand-alone reverse transcriptase. The highly concentrated and glycerol-free formulation makes the product suitable for lyophilization and for the preparation of dry amplification mixes.
Improve sensitivity and specificity
- high thermostability - no loss of activity even at the reaction temperature higher than +65°C for improved handling of secondary structures and difficult targets.

Easily lyophilize your assays
-high concentration (500 U/uL) and glycerol-free formulation allowing easy lyophilization and drying down.

Stay specific
- Make use of the wide temperature activity range of NxtScript 2G RT and reverse transcribe at the temperature that is optimal for your RNA target.
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Application
Use NxtScript 2G RT for synthesis of cDNA from total RNA or mRNA for:
Two-step and one-step RT-PCR applications
RT-PCR for detection of viral targets
RT-PCR for detection of mRNA targets, such as cancer biomarkers.
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Specification
NxtScript 2G reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Recommended reaction temperature: +55 to +70°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg2+
Appearance: Clear, colourless solution
Volume activity: > 500 U/μL
Nicking activity (pBR 322 DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
RNases (MS2 RNA): Not detectable in up to 75 U after 1 hour incubation at +37°C.
E. coli DNA: ≤ 100 pg E.coli DNA/mL enzyme
Human DNA: ≤ 15 ng human DNA/mL enzyme
Performance test: TwoStep RT-qPCR, β-Actin (on LC480 II with human reference RNA and β-Actin assay)
Stability: At -15 to -25°C within specification range for 12 months.