For further processing only. Others NxtScript RT high conc. 1MU NxtScript Reverse Transcriptase, high conc., 1MU 3.6.14.3.11.3 mutant from Moloney Murine Leukemia Virus, expressed in E.coli 09917225001 NxtScript RT, high conc., 1MU NxtScript RT, high conc., 1MU Reagents, kits 200 µl  Not Available Will be supplied as "NxtScript RT, high conc., 1MU". Unit of Measure is "PC".1 PC contains 200 µl EC 2.7.7.49 en Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:One-step and two-step RT-PCR applicationsRT-PCR for detection of viral targetsRT-PCR for detection of mRNA targets, such as cancer biomarkersTMA and NASBA nucleic acid amplification methodsGeneration of full-length cDNA librariesRapid amplification of cDNA ends (RACE) en NxtScript RT high. Conc. Is a stand-alone reverse transcriptase. The highly concentrated formulation makes the product more suitable for lyophilization and for the preparation of dry amplification mix preparations. en Highly concentrated M-MLV Reverse Transcriptase mutant designed for high thermostability. en Reverse transcribe difficult templates.The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).Achieve higher yield.NxtScript RT lacks RNase H activity. This results in higher cDNA yields.Stay specific.Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target. en Appearance: Clear, colorless solutionActivity (calculated): 5525 - 6800 kU /mlProtein concentration: 13 - 16 mg / mlFunction test one-step RT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH Assay)Purity (SDS-PAGE): ≥90%Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.Stability: At -15 to -25°C within specification range for 30 months. en