For further processing only. Others NxtScript 2G RT conc. NxtScript 2G RT, conc. 3.6.14.3.11.4 thermostable reverse transcriptase, 500 U/μL, glycerol-free solution NxtScript 2G RT, conc. custom fill500 U/μL CustomBiotech product. Please contact your local representative. Will be supplied as "NxtScript 2G RT, conc.". Unit of Measure is "kU". NxtScript 2G RT, conc. 09085220103 Not Available Reagents, kits en Highly concentrated, glycerol-free reverse transcriptase developed for high temperature reactions en NxtScript 2G RT, conc. is a stand-alone reverse transcriptase. The highly concentrated and glycerol-free formulation makes the product suitable for lyophilization and for the preparation of dry amplification mixes. en Use NxtScript 2G RT for synthesis of cDNA from total RNA or mRNA for:Two-step and one-step RT-PCR applicationsRT-PCR for detection of viral targetsRT-PCR for detection of mRNA targets, such as cancer biomarkers. en Improve sensitivity and specificity- high thermostability - no loss of activity even at the reaction temperature higher than +65°C for improved handling of secondary structures and difficult targets.Easily lyophilize your assays-high concentration (500 U/uL) and glycerol-free formulation allowing easy lyophilization and drying down.Stay specific- Make use of the wide temperature activity range of NxtScript 2G RT and reverse transcribe at the temperature that is optimal for your RNA target. en Appearance: Clear, colourless solutionVolume activity: > 500 U/μLNicking activity (pBR 322 DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.RNases (MS2 RNA): Not detectable in up to 75 U after 1 hour incubation at +37°C.E. coli DNA: ≤ 100 pg E.coli DNA/mL enzymeHuman DNA: ≤ 15 ng human DNA/mL enzymePerformance test: TwoStep RT-qPCR, β-Actin (on LC480 II with human reference RNA and β-Actin assay)Stability: At -15 to -25°C within specification range for 12 months.