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Two-step and one-step RT-PCR applications
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RT-PCR for detection of mRNA targets, such as cancer biomarkers.", "Name": "Applications" }, { "Language": "en", "Value": "Improve sensitivity and specificity
- high thermostability - no loss of activity even at the reaction temperature higher than +65°C for improved handling of secondary structures and difficult targets.

Easily lyophilize your assays
-high concentration (500 U/uL) and glycerol-free formulation allowing easy lyophilization and drying down.

Stay specific
- Make use of the wide temperature activity range of NxtScript 2G RT and reverse transcribe at the temperature that is optimal for your RNA target.", "Name": "Benefits" }, { "Language": "en", "Value": "Will be supplied as \"NxtScript 2G RT, conc.\". Unit of Measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "NxtScript 2G reverse transcriptase is high thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
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Nicking activity (pBR 322 DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
RNases (MS2 RNA): Not detectable in up to 75 U after 1 hour incubation at +37°C.
E. coli DNA: ≤ 100 pg E.coli DNA/mL enzyme
Human DNA: ≤ 15 ng human DNA/mL enzyme
Performance test: TwoStep RT-qPCR, β-Actin (on LC480 II with human reference RNA and β-Actin assay)
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" } ] } } ] }

NxtScript 2G RT, conc.

thermostable reverse transcriptase, 500 U/μL, glycerol-free solution

Heat-powered RT-PCR performance in a new generation

The NxtScript family is a designed mutated reverse transcriptase cluster selectively evolved toward thermostability at increasingly higher temperatures.

NxtScript 2G RT is the latest member of the family and pushes the temperature of the RT step to new levels: no loss of activity even at 70°C. At this reaction temperature, common restrictions of RT-PCR are removed, simplifying assay design and eliminating compromises


  • Easily detect difficult RNA targets
    At higher temperatures, RNA secondary structures unravel and no longer prevent amplification.
  • Tackle any GC-rich regions
    The thermostability of the enzyme no longer limits melting temperature
  • Work with any target of interest
    Make no compromises in your RT-PCR design because of target sequence.

Great creations need premium ingredients

Reagents made for IVD assays

Today’s molecular diagnostics are more than a target and an assay. They operate in a fast-paced, demanding setting, delivering accurate data on time.

Such creations are forged from reagents that empower performance and possibilities.



Technical documents

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