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"Language": "en",
"Value": "Antibody-mediated hot start 2nd generation mutant of Taq, specifically designed for fast PCR.",
"Name": "Positioning"
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{
"Language": "en",
"Value": "The kit contains 2 vials of antibody-mediated hot start DNA polymerase and all buffers necessary to optimize the amplification reaction.",
"Name": "Product Description"
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"Language": "en",
"Value": "Will be supplied as \"KAPA2G Fast HotStart PCR Kit\". Unit of measure is \"piece\".",
"Name": "CB - Order Information"
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"Value": "
Save valuable time. Reach extension times as low as 1 sec/kb and reduce PCR reaction times by up to 75%.
Work with difficult templates Cover a broad range of both AT- and GC-rich targets.
",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "Fast amplification of DNA fragments up to 3 kb in PCR assays:
Fast PCR
Routine PCR
Genotyping
",
"Name": "Applications"
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{
"Language": "en",
"Value": "↵5 U/μL KAPA2G Fast HotStart DNA Polymerase
2 x 500 μL tube
5X KAPA2G Buffer A
2 x 30 mL bottle
25 mM MgCl2
2 x 10 mL bottle
",
"Name": "Content"
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{
"Language": "en",
"Value": "Volume activity: 5 U/μL Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C. Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C. Tests for the presence of contaminating nucleic acids (E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification (human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification Performance test (≥ 1 ng DNA): Corresponds to specification",
"Name": "Specification"
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"Value": "KAPA2G Fast HotStart DNA Polymerase is a second-generation enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA Polymerase. In addition to speed, it provides higher yield and sensitivity than competitor enzymes across a broad range of targets.",
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KAPA2G Fast HotStart PCR Kit
mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer