{ "ProductData": { "ID": "3.9.1.1.1.2", "ProductType": "Others", "BrandName": "KAPA2G Fast HotStart PCR Kit", "ProductNameAddition": "mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "KAPA2G Fast HotStart PCR Kit" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "211", "StructureNodeStatus": "valid", "NodeName": "KAPA" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-042-00", "StructureNodeStatus": "valid", "NodeName": "DNA Polymerases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0136", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "320-00", "StructureNodeStatus": "valid", "NodeName": "Labeling and Detection" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "08041202001", "MaterialDescription": "KAPA2G Fast HotStart PCR Kit", "RegisteredProductName": "KAPA2G Fast HotStart PCR Kit", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "KK0010", "PackSizePIM360": "5000 U", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"KAPA2G Fast HotStart PCR Kit\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Positioning", "Value": "Antibody-mediated hot start 2nd generation mutant of Taq, specifically designed for fast PCR.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Product Description", "Value": "The kit contains 2 vials of antibody-mediated hot start DNA polymerase and all buffers necessary to optimize the amplification reaction.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"KAPA2G Fast HotStart PCR Kit\". Unit of measure is \"piece\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Benefits", "Value": "

• Save valuable time.
  Reach extension times as low as 1 sec/kb and reduce PCR reaction times by up to 75%.
• Work with difficult templates
  Cover a broad range of both AT- and GC-rich targets.

", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Applications", "Value": "Fast amplification of DNA fragments up to 3 kb in PCR assays:

• Fast PCR
• Routine PCR
• Genotyping

", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Content", "Value": "  ↵5 U/μL KAPA2G Fast HotStart DNA Polymerase

• 2 x 500 μL tube

5X KAPA2G Buffer A

• 2 x 30 mL bottle

25 mM MgCl₂

• 2 x 10 mL bottle

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Specification", "Value": "Volume activity: 5 U/μL  
Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids
(E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 1 ng DNA): Corresponds to specification", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "KAPA2G Fast HotStart DNA Polymerase is a second-generation enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA Polymerase. In addition to speed, it provides higher yield and sensitivity than competitor enzymes across a broad range of targets.", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }