{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "KAPA2G Fast HotStart PCR Kit", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "KAPA2G Fast HotStart PCR Kit", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.9.1.1.1.2", "ProductNameAddition": "mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Antibody-mediated hot start 2nd generation mutant of Taq, specifically designed for fast PCR.", "Name": "Positioning" }, { "Language": "English", "Value": "The kit contains 2 vials of antibody-mediated hot start DNA polymerase and all buffers necessary to optimize the amplification reaction.", "Name": "Product Description" }, { "Language": "English", "Value": "Will be supplied as \"KAPA2G Fast HotStart PCR Kit\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "2 x 500 μL tube of 5 U/μL KAPA2G Fast HotStart
2 x 30 mL tube of 5x 2G Buffer A
2 x 10 mL tube of 25 mM MgCl₂", "Name": "Content (Catalog)" }, { "Language": "English", "Value": "

• Save valuable time.
  Reach extension times as low as 1 sec/kb and reduce PCR reaction times by up to 75%.
• Work with difficult templates
  Cover a broad range of both AT- and GC-rich targets.

", "Name": "Benefits" }, { "Language": "English", "Value": "Volume activity: 5 U/μL  
Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids
(E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 1 ng DNA): Corresponds to specification", "Name": "CB - Specification" }, { "Language": "English", "Value": "Fast amplification of DNA fragments up to 3 kb in PCR assays:

• Fast PCR
• Routine PCR
• Genotyping

", "Name": "Applications" }, { "Language": "English", "Value": "KAPA2G Fast HotStart DNA Polymerase is a second-generation enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA Polymerase. In addition to speed, it provides higher yield and sensitivity than competitor enzymes across a broad range of targets.", "Name": "CB - Properties" }, { "Language": "English", "Value": "Volume activity: 5 U/μL  
Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids
(E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 1 ng DNA): Corresponds to specification", "Name": "Specification" }, { "Language": "English", "Value": "KAPA2G Fast HotStart DNA Polymerase is a second-generation enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA Polymerase. In addition to speed, it provides higher yield and sensitivity than competitor enzymes across a broad range of targets.", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Family", "NodeName": "KAPA", "StructureSystemID": null, "NodeID": "211" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->DNA Polymerases", "StructureSystemID": null, "NodeID": "70-042-00" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "08041202001", "MaterialDescription": "KAPA2G Fast HotStart PCR Kit", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "KK0010", "PackSizePIM360": "5000 U", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"KAPA2G Fast HotStart PCR Kit\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }