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On-board stability: After first use on the instrument, the reagent is stable for up to 12 weeks.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Store the reagent cassette at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator D Bio*
  • Control D Level 1 Bio*
  • Control D Level 2 Bio*
  • Control D Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Measuring Range
Acetate concentration can be determined in following ranges:
  • AC2B: 10 - 2300 mg/L (0.17 - 38.9 mmol/L)
  • AC2D: 800 - 23,000 mg/L (14 - 389 mmol/L),
up to 230 g/L (3.89 mol/L) with automatic dilution

Conversion Factors
Conversion factors for acetate concentration:
  • 1 mmol/L = 59.05 mg/L
  • 1 g/L = 16.93 mmol/L

Traceability
This method has been standardized against an independent photometric ezymatic assay.

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).

 Level 1Level 2Level 3
Mean2.79 mmol/L  
(165 mg/L)
17.83 mmol/L\t 
(1053 mg/L)
28.41 mmol/L
(1678 mg/L)
CV in-run\t\t 1.6 %1.0 %0.7 %
CV inter-run4.5 %3.9 %4.0 %
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "This product is intended for quantitative determination of acetate in aqueous solutions using the Cedex Bio HT Analyzer.", "Name": "Product Description" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
VialContentComposition
AC2BReagent cassette,
200 tests
Reagent 1 (R1): Triethanolamine, 0.2 mol/L; L-malic acid, 2.4 g/L; magnesium chloride, 1.2 g/L; coenzyme A, 0.3 g/L; adenosine triphosphate, 3.8 g/L; nicotinamide adenine dinucleotide, 3 g/L; dithiothreitol, 0.3 g/L; preservative; pH 4.8.
Start Reagent (SR): Potassium phosphate buffer, 100 mM, pH 9.0; L-malate dehydrogenase, 119 U/mL; citrate synthase, 28 U/mL; acetate kinase, 32.5 U/mL; phosphotransacetylase, 97 U/mL; stabilizer; preservative.
", "Name": "Content" }, { "Language": "en", "Value": "Application AC2B (068), AC2D (082)
​​​​​​​

Cedex Bio HT test definition:
Measuring mode\t\t\t\t\t  Absorbance
Abs. calculation mode\t\t\t Endpoint
Reaction direction\t\t\t\t\tIncrease
Wavelength A/B\t\t\t\t\t\t340/409 nm
Calc. first/last\t\t\t\t\t\t\t39/69
Unit\t\t\t\t\t\t\t\t\t\t\t mmol/L
Reaction mode\t\t\t\t\t\tR1-S-SR

Pipetting parameters:
R1\t\t\t\t\t\t\t\t\t\t\t  120 μL + 5 μL Dilutent (H2O)
Sample\t\t\t\t\t\t\t\t\t\t 2 μL + 5 μL Dilutent (H2O)
SR\t\t\t\t\t\t\t\t\t\t\t   30 μL + 5 μL Dilutent (H2O)
Total volume\t\t\t\t\t\t   167 μL

Calibration:
Calibrator\t\t\t\t\t\t\t\t Calibrator D Bio (CALD,07-1141-1)
Calibration mode\t\t\t\t\tLinear regression
Calibration interval\t\t\t\t 56 days, and if recalibration is required due to QC results

Quality control:
Controls
Control D Level 1 Bio (COND1, 07-1143-8)
Control D Level 2 Bio (COND2, 07-1144-6)
Control D Level 3 Bio (COND3, 07-1145-4)
Use the recommended control material. Other suitable control material can be used in addition.
Control intervals and acceptance limits should be adapted to each laboratory's individual requirements.
If values do not fall within the defined limits, corrective measures and recalibration are required", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "Acetate is an important metabolyte in microbial fermentation. Accumulation of acetate can suppress culture growth and product formation. For monitoring in a culture process, acetate can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using phosphorylation by acetate kinase.
Recovery: ±15%", "Name": "Specification" } ] } } ] }

Acetate V2 Bio HT

Test kit for the Cedex Bio HT Analyzer

acetate

Overview

Ordering

Technical documents

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