KAPA3G HotStart DNA Polymerase, Glycerol-free, 30 U/µL
from Thermus aquaticus, expressed in E. coli
For further processing only.
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KAPA3G HotStart DNA Polymerase, Glycerol-free, 30 U/µL material number and pack size: Material Number Pack Size 08918651103 custom fill 30 U/μL
- Kapa3G HotStart DNA Poymerase is an antibody-mediated HotStart 3rd generation mutant of Taq specifically designed for fast and inhibitor-resistant PCR and formulated without glycerol for preparation of dried amplification mixes. The product consists of antibody-mediated KAPA3G HotStart DNA polymerase at 30 U/µL.
- Fast and robust DNA amplification out of samples containing PCR inhibitors.
KAPA3G HotStart DNA Polymerase is a highly inhibitor resistant Taq mutant that reduces effort and time for DNA purification, enabling streamlined sample-to-result workflows is a second-generation Taq polymerase engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq. In addition to speed, it provides consistent amplification across a broad range of amplicon types (GC- and AT-rich), eliminating the need for optimization using multiple enzymes and protocols. It enables robust PCR performance, high sensitivity, and improved tolerance to common inhibitors such as NaCl, EtOH, SDS and Urea.
Volume activity: ≥ 30 U/μL
Unspecific endonucleases (plasmid DNA): Not detectable up to 10 U using pBR 322 DNA/16 hours/ +37°C
Exonucleases: Not detectable up to 10 U using
λ-DNA / 16 hours / +37°C
E. coli DNA: ≤ 100 pg E.coli DNA/mL enzyme
Human DNA: ≤ 15 ng human DNA/mL enzyme