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KAPA Express Extract

For further processing only.

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Order information
KAPA Express Extract material number and pack size:
Material Number Pack Size
08041253001 1000 reactions
Will be supplied as "KAPA Express Extract". Unit of measure is "piece".
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Overview
Fast and simple extraction of DNA for short PCR workflows in combination with PCR inhibitor-resistant DNA Masters.
DNA extraction kit includes protease mix and extraction buffer.
  • Shorten the time to prepare your sample. A fast and simple protocol allows you to have the DNA PCR-ready in 15 minutes.
  • Be flexible. The kit works with a variety of sample types.
  • Reduce risk of contamination. Work only with single-tube reactions.
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Application
Extraction of PCR-ready DNA from, but not limited to, the following sample types:
  • Human tissue (FFPE samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples)
  • Animal samples (ear or tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
  • Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples)
  • Insects (crushed)
  • Bird feathers (calamus fragments)

KAPA Express Extract gives best results when used together with PCR inhibitor-resistant DNA Masters such as KAPA2G Robust HotStart ReadyMix or KAPA3G HotStart Master.

Note: KAPA Express Extract is not suitable for DNA extraction from plants; for plant PCR applications, the KAPA3G Plant PCR Kit (08 041 091 001) is recommended.
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Specification
KAPA Express Extract is a thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from various tissue types in as little as 15 minutes. It is designed for optimal tissue lysis and sample preservation. Unlike protocols that rely on proteinase K digestion, DNA extractions with KAPA Express Extract are conveniently performed in a single tube, without the need for hazardous chemicals and multiple washing steps. This greatly reduces the risk of sample loss and contamination.
Volume activity: 1 U/μL  
Tests for the presence of contaminating nucleic acids
(E.coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification