Galactose/Arabinose Bio HT
Test kit for the Cedex Bio HT Analyzer
For use in quality control / manufacturing process only.
Galactose/Arabinose Bio HT material number and pack size: Material Number Pack Size 08391629001 200 tests
- Kit for determination of galactose and arabinose. This product is intended for quantitative determination of galactose or arabinose in aqueous solutions using the Cedex Bio HT Analyzer.
- Galactose: In the production of therapeutic proteins using CHO cell cultures, replacement of glucose by galactose can be used to control the glycosylation pattern of the product, especially to maximize the n-glycan galactosylation level. For control of culture media and for monitoring in the culture process, the galactose concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.
Arabinose: Continuous monitoring of the arabinose concentration is required in the process control for E. coli cultures with a pBAD promoter for recombinant protein production. The arabinose concentration needs to be exactly adjusted by appropriate feeding, in order to control the dose-dependent induction of the protein expression and to provide sufficient nutrition to achieve the optimal yield. Using a Cedex Bio or Cedex Bio HT Analyzer, the arabinose concentration can be determined fast and accurately.
Method: Enzymatic photometric assay using galactose dehydrogenase
Recovery: ± 15%