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For use in quality control/manufacturing process only. Others Acetate V2 Bio Acetate V2 Bio 3.8.10.2.4.20 Test kit for the Cedex Bio Analyzer Acetate V2 Bio 07613336130643 4 x 50 tests CustomBiotech product. Please contact your local representative. true Unit of measure is "piece". Acetate V2 Bio 07395442001 Not Available Reagents, kits en Acetate (or acetic acid) is converted to acetyl-CoA using ATP and CoA in presence of acetate kinase (AK) and phosphotransacetylase (PTA). Acetyl-CoA reacts with oxaloacetate in presence of citrate synthase (CS) to citrate. The required oxaloacetate is produced by oxidation of L-malate with NAD+ in presence of L-malate dehydrogenase (MDH), but only if the oxaloacetate is immediately consumed by acetyl-CoA, otherwise the reverse reaction of oxaloacetate to L-malate would be the dominant direction. The NADH generated in the oxaloacetate production is measured by increase in absorbance at 340 nm, and this relates to the formation of Acetyl-CoA which directly depends on the acetate concentration of the sample. en This product is intended for quantitative determination of acetate in aqueous solutions using the Cedex Bio Analyzer*. en Acetate is an important metabolyte in microbial fermentation. Accumulation of acetate can suppress culture growth and product formation. For monitoring in a culture process, acetate can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience. en Method: Enzymatic photometric assay using phosphorylation by acetate kinase. Recovery: ±15%