from Streptomyces avidinii, expressed in E. coli, liquid
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Streptavidin, recombinant material number and pack size: Material Number Pack Size 08903727103 0.5GAI, 5GAI
- Use Streptavidin, recombinant as a tool for solid phase technology and universal detection systems in immunology and molecular diagnostics.
Streptavidin consists of four subunits with a molecular weight of 13 kD, each containing a single biotin binding site. Each subunit has six thyrosine residues. The protein is carbohydrate free.
Streptavidin + 4 biotin -> streptavidin · (biotin)4
The formation of the complex is measured at 233 nm. Appearance: Clear, colourless solution
Specific activity: ≥ 17 units/mg protein
Protein concentration (abs. 282 nm; factor 3.1) 70 ± 5 mg/mL
Proteases (Azokoll F; 24 h, +25°C) ≤ 0,001 U/mg Protein corresponds
SDS-PAGE (homogeneous): 70416 corresponds
Stability: At -15° to -25°C within specification range for 12 months.