Alkaline Phosphatase, recombinant, highly active
from calf intestine, expressed in Pichia pastoris, solution
For further processing only.
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Alkaline Phosphatase, recombinant, highly active material number and pack size: Material Number Pack Size 03137031103 custom fill
- Marker enzyme for preparation of antibody- / antigen-enzyme conjugates incorporated in highly sensitive immunoassay reagents for colorimetric, fluorimetric and luminometric detection. Alkaline Phosphatase is recommended for conjugation via carbohydrate groups (content approximately 30%).
IEP (IEF, CE): 3.6-4.7
Total molecular weight: 124±10 kD
Molecular weight protein: 104 kD (=84%)
Molecular weight carbohydrate: 20±10 kD (16±6%)
Accessible N-glycosylation sites: 2/subunit
Branching type: Higher branched (hybrid) type GlcNAc, Mannose, no NeuAc detected
O-glycosylation sites: Not detected
Number of isoenzymes:
Present: 3 (MS)
Based on protein: 1 (MS) Appearance: Clear, colorless solution in NaCl, 3 mol/L; MgCl2, 5 mmol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L, pH approximately 7.6
pH value: 7.0-8.0
Protein (A280, 1 mg/mL=1, against water): 20±1 mg/mL
Specific activity (+37°C, 4-NPP): ≥7,000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 5-13 mol/mol
Carbohydrates, n=2: No limit
Stability: At +2 to +8°C within specification range for 12 months.