• Broad spectrum

    of products for

    your industry

Next
Prev

Expand High Fidelity PCR Buffer

10x conc., with MgCl2

For further processing only.

expand | collapse
+-
Order information
Expand High Fidelity PCR Buffer material number and pack size:
Material Number Pack Size
05917131103 1 mL 1 piece contains 1 ml
Will be supplied as "Exp.HF Buffer 10x w MgCl2 MPB". Unit of measure is "piece".
+-
Overview
Standard reaction buffer for PCR using the Expand High Fidelity PCR System.
+-
Application
Use this buffer together with Expand High Fidelity PCR System.
+-
Specification
Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 220 mmol/L; MgCl2, 15 mmol/L; pH approximately 8.9 at +25°C
Unspecific endonucleases (λDNA and MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.