For further processing only. Others Expand High Fidelity PCR Buffer Expand High Fidelity PCR Buffer 3.6.6.4.59.1 10x conc., with MgCl2 05 917 131 103 5 917 131 103 05917131103 5917131103 05917131103 Expand HF Buffer 10x with MgCl2 Expand High Fidelity PCR Buffer 07613336131589 Reagents, kits 1 mL 1 piece contains 1 mL CustomBiotech product. Please contact your local representative. Will be supplied as "Exp.HF Buffer 10x w MgCl2 MPB". Unit of measure is "piece". Use this buffer together with Expand High Fidelity PCR System. en Standard reaction buffer for PCR using the Expand High Fidelity PCR System. en Appearance: Clear, colorless solutionContents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 220 mmol/L; MgCl2, 15 mmol/L; pH approximately 8.9 at +25°CUnspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.Function test in PCR (human genomic DNA, 4.8 kb tPA fragment): Corresponds to specificationStability: At -15 to -25°C within specification range for 24 months. en

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