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dUTP, PCR Grade

sodium salt, 100 mM

For further processing only.

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Order information
dUTP, PCR Grade material number and pack size:
Material Number Pack Size
11889532103 100 ml 10,000 μmol
04631145103 20 ml 2,000 μmol
11889532103: Will be supplied as ''dUTP, Na, Solution (PCR Grade)''. Unit of measure is ''μmol''.
04631145103: Will be supplied as ''dUTP PCR Grade, Sodium Solution, 20 mL''. Unit of measure is ''μmol''.
GMP-manufactured dNTP's from the leading manufacturer of nucleotides for outstanding, consistent performance in amplification reactions.
dUTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
dUTP, PCR Grade, is designed for amplification reactions where high-quality reagents are required, such as for in vitro diagnostics. Avoid DNA carryover contamination between PCRs that can be a source of false positives.
To decontaminate PCR and RT-PCR mixes, dUTP is incorporated in place of dTTP into the PCR product. Reaction mixes are then pre-treated with Uracil-DNA Glycosylase (UNG) before amplification to degrade contaminating dUTP-containing PCR products.
Nomenclature: 2'-Deoxy-uridine-5'-triphosphate
Formula: C9H15N2O14P3
Molecular weight: 468.2 D
Appearance: Clear, colorless solution
pH value: 8.1-8.5
dUTP (1 μmol ≙ 9.9 A260 units, pH 7.0): 100-110 mmol/L
dUTP (high resolution HPLC method): ≥99 area%
dUDP (HPLC): ≤0.9 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.74±0.02
A280/A260: 0.38±0.02
A290/A260: 0.04±0.01
Function test in RT-PCR (RNA, human dystrophin, and mouse β-actin gene): Corresponds to specification
Stability: At -15 to -25°C within specification range for 42 months.