FastStart PCR Buffer
10x conc., without MgCl2
For further processing only.
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Will be supplied as "PCR buffer (10X) w/o MgCl2". Unit of measure is "mL".
FastStart PCR Buffer material number and pack size: Material Number Pack Size 12161494103 custom fill 05917166103 1 mL - Standard reaction buffer without MgCl2 for optimization of the MgCl2 concentration in PCR using FastStart Taq DNA Polymerase.
- Use this buffer together with FastStart Taq DNA Polymerase whenever the amplification of difficult target requires a specific MgCl2 concentration.
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Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 50 mmol/L; KCl, 100 mmol/L; pH approximately 8.3 at +25°C
Unspecific endonucleases (λDNA, MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activitiy (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR:
10 ng human genomic DNA, 4.8 kb tPA fragment/ 1.8 kb EPO fragment: Corresponds to specification
50 pg human genomic DNA, 365 bp tPA fragment: Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.