FastStart Taq DNA Polymerase, 5 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution
For further processing only.
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FastStart Taq DNA Polymerase, 5 U/μl material number and pack size: Material Number Pack Size 12161508103 custom fill
The enzyme is supplied without reaction buffer.
Hot start Taq DNA Polymerase for highly specific and sensitive amplification using PCR.
Achieve high specificity, sensitivity, and yield.
Prevent the extension of non-specifically bound primers using this hot start enzyme.
- For applications see FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl
See FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl
Appearance: Clear to slightly opalescent, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20; 0.2% (v/v); glycerol, 50% (v/v); pH approximately 9.0 at +25°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 15 U after 4 hours incubation at +65°C.
Function test in PCR
(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(200 ng human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.