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Taq DNA Polymerase, 50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
Taq DNA Polymerase, 50 U/μl material number and pack size:
Material Number Pack Size
04827007103 custom fill
Will be supplied as "Taq DNA Pol., Glycerol-free". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
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Overview
Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes.
High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.
Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience.
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Application
Use Taq DNA Polymerase, 50 U/μl, especially for:
  • Setup of PCR master mixtures, when highly concentrated components are required
  • Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature

For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl
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Specification
See Taq DNA Polymerase, GMP Grade, 5 U/μl
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Glycerol content: ≤0.1% (v/v)
Volume activity: 55±5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.