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Taq DNA Polymerase, 50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

For further processing only.

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Order information
Taq DNA Polymerase, 50 U/μl material number and pack size:
Material Number Pack Size
04827007103 custom fill
Will be supplied as "Taq DNA Pol., Glycerol-free". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
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Overview
Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes.
High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.
Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience.
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Application
Use Taq DNA Polymerase, 50 U/μl, especially for:
  • Setup of PCR master mixtures, when highly concentrated components are required
  • Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature

For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl
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Specification
See Taq DNA Polymerase, GMP Grade, 5 U/μl
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Glycerol content: ≤0.1% (v/v)
Volume activity: 55±5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.
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License disclaimer

48:Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.