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AptaTaq DNA Polymerase LDx, 5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
AptaTaq DNA Polymerase LDx, 5 U/μl material number and pack size:
Material Number Pack Size
05884314103 custom fill 5 U/μl
Will be supplied as "AptaTaq DNA Polymerase LDx, 5 U/μL". Unit of measure is "kU".
Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity in microbial testing.
AptaTaq DNA Polymerase LDx is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for applications detecting lowest levels of DNA.
  • Minimize risks from contaminating nucleic acids.
    AptaTaq DNA Polymerase LDx is extensively tested using ultra sensitive tests for contaminating nucleic acids from bacteria and fungi. Roche has developed a nucleic acid-free workflow with clearly defined, highly consistent manufacturing processes to offer a product with very low nucleic acid background.
  • Enjoy the benefits of the advanced AptaTaq hot start system.
    Use AptaTaq DNA Polymerase for additional benefits including speed, easy handling and consistent results.
Select AptaTaq DNA Polymerase LDx to perform microbial testing and other assays where the absence of contaminating bacterial, fungal, and/or human DNA is crucial. AptaTaq DNA LDx Polymerase is ideal for:
  • Fast PCR assays with no extra enzyme activation time and fast cycling protocols
  • Single- and multiplex PCR and qPCR applications that require high specificity, sensitivity, and yield
  • RT-PCR
  • Difficult templates with secondary structures or GC-rich sequences
  • Automated PCR workflows requiring high stability of the reaction mixtures during automated pipetting and prolonged handling at room temperature
AptaTaq DNA Polymerase LDx is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase that lacks 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum for elongation: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg2+ (standard concentration, 1.5 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C
Volume activity: 5.5±0.5 U/μL
Aptamer concentration (HPLC): 3.58 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Tests for the absence of contaminating nucleic acids
(human genomic DNA, β-Globin fragment, ≤3 positve of 15 samples): Corresponds to specification
(LightCycler® UniTOOL ResoLight assay, detecting grampositive and gramnegative bacterial DNA and fungal DNA, <1.0 copy genomic DNA/20 U enzyme): Corresponds to specification
Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.