AptaTaq exo DNA Polymerase, 5 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution
For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.
AptaTaq exo DNA Polymerase, 5 U/μl material number and pack size: Material Number Pack Size 05458030103 custom fill 5 U/μl
N-terminal truncated Taq DNA Polymerase with reversible hot start system and no 5'-3' exonuclease activity for optimal detection of mismatches.
This novel optimized mixture of high-quality N-terminal-deleted Taq DNA Polymerase and a specific oligonucleotide (aptamer) provides improved discrimination against misextension. As with the AptaTaq DNA Polymerase System, the AptaTaq exo DNA Polymerase-based assay shows high specificity and a broad dynamic range of products.
- Optimize your SNP analysis.
Discriminate between paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
- Obtain reliable results fast.
Benefit from the general features of the AptaTaq DNA Polymerase System with the differentiating capabilities of a 5'-3' exonuclease activity-lacking Taq DNA Polymerase.
- Optimize your SNP analysis.
- Use AptaTaq exo DNA Polymerase for:
- SNP analysis and genotyping
- Allele-specific PCR
- Arbitrarily primed PCR
- Automated PCR requiring prolonged handling at room temperature
When time to result matters, this novel hot start technology is ideal as it does not require any activation time.
AptaTaq exo DNA Polymerase is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase that lacks 5'-3' and 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 8.3 (+20°C)
Temperature optimum for elongation: Approximately +72°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg2+ (standard concentration, 2 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); casein, 0.1 g/L; pH approximately 8.0 at +4°C
Volume activity: 5.5±0.5 U/μL
Aptamer concentration (HPLC): 24.0 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.