AptaTaq exo DNA Polymerase, 50 U/μl
from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution
For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.
AptaTaq exo DNA Polymerase, 50 U/μl material number and pack size: Material Number Pack Size 05364086103 custom fill 50 U/μl
N-terminal truncated Taq DNA Polymerase with reversible hot start system and no 5'-3' exonuclease activity for optimal detection of mismatches; lyo ready formulation for preparation of dried amplification mixes.
AptaTaq DNA exo Polymerase is a blend of N-terminal truncated Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for excellent discrimination against misextension. This concentrated formulation contains no glycerol and is suitable for the preparation of dried-down amplification mix preparations.
- Optimize your SNP analysis.
Discriminate between paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
- Obtain reliable results fast.
Benefit from the general features of the AptaTaq DNA Polymerase System with the discriminating capabilities of a 5'-3' exonuclease activity-lacking Taq DNA Polymerase.
- Prepare stable amplification mixes in dry format.
Use this formulation for producing dried-down amplification mixes stable at room temperature.
- Optimize your SNP analysis.
- Use AptaTaq exo DNA Polymerase for:
- SNP analysis and genotyping
- Allele-specific PCR
- Arbitrarily primed PCR
- Formulation of dried-down amplification reagents
When time to result matters, this novel hot start technology is ideal as it does not require any activation time.
AptaTaq exoDNA Polymerase is active at temperatures above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase itself is a highly processive 5'-3' DNA Polymerase lacking 5'-3' and 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 8.3 (+20°C)
Temperature optimum for elongation: Approximately +72°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg2+ (standard concentration, 2 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L;EDTA, 0.1 mmol/L; DTT, 1 mmol/L; casein 1 g/L; glycerol-free; pH approximately 8.0 at +4°C
Volume activity: 55±5 U/μL
Aptamer concentration (HPLC): 240 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Function test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.