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AptaTaq exo DNA Polymerase, 50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
AptaTaq exo DNA Polymerase, 50 U/μl material number and pack size:
Material Number Pack Size
05364086103 custom fill 50 U/μl
Will be supplied as "AptaTaq exo DNA Polymerase, Glyc.-free". Unit of measure is "kU".
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Overview
N-terminal truncated Taq DNA Polymerase with reversible hot start system and no 5'-3' exonuclease activity for optimal detection of mismatches; lyo ready formulation for preparation of dried amplification mixes.
AptaTaq DNA exo Polymerase is a blend of N-terminal truncated Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for excellent discrimination against misextension. This concentrated formulation contains no glycerol and is suitable for the preparation of dried-down amplification mix preparations.
  • Optimize your SNP analysis.
    Discriminate between paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
  • Obtain reliable results fast.
    Benefit from the general features of the AptaTaq DNA Polymerase System with the discriminating capabilities of a 5'-3' exonuclease activity-lacking Taq DNA Polymerase.
  • Prepare stable amplification mixes in dry format.
    Use this formulation for producing dried-down amplification mixes stable at room temperature.
     
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Application
Use AptaTaq exo DNA Polymerase for:
  • SNP analysis and genotyping
  • Allele-specific PCR
  • Multiplexing
  • Arbitrarily primed PCR
  • Formulation of dried-down amplification reagents

When time to result matters, this novel hot start technology is ideal as it does not require any activation time.
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Specification
AptaTaq exoDNA Polymerase is active at temperatures above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase itself is a highly processive 5'-3' DNA Polymerase lacking 5'-3' and 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 8.3 (+20°C)
Temperature optimum for elongation: Approximately +72°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg2+ (standard concentration, 2 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L;EDTA, 0.1 mmol/L; DTT, 1 mmol/L; casein 1 g/L; glycerol-free; pH approximately 8.0 at +4°C
Volume activity: 55±5 U/μL
Aptamer concentration (HPLC): 240 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Function test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.