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Taq DNA Polymerase, GMP Grade, 5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
Taq DNA Polymerase, GMP Grade, 5 U/μl material number and pack size:
Material Number Pack Size
03707628103 5 kU 5 U/μl
03161455103 50 kU 5 U/μl
03707628103: Will be supplied as "Taq DNA Polymerase Ind. GMP Grade, 5 kU". Unit of measure is "piece".
03161455103: Will be supplied as "Taq DNA Polym GMP Grade 50ku". Unit of measure is "piece".
The enzyme is supplied without reaction buffer.
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Overview
Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.
  • Obtain consistent results.
    Rely on the robust reaction performance and the lot-to-lot consistency of this product.
  • Stay ahead of regulatory requirements.
    High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations.
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Application
Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:
  • Routine PCR and RT-PCR applications
  • Amplification of DNA fragments up to 3 kb from various sources of DNA
  • Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
  • Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)
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Specification
Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C.
Volume activity: ≥5 U/μL
Specific activity (Protein: A280): ≥130,000 U/mg
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Purity (SDS PAGE): ≥98%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 10 U after 1 hour incubation at +37°C.
Function test in PCR (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Function test in qPCR using LightCycler®
(human genomic DNA, β-globin gene): Corresponds to reference
(plasmid DNA, β-globin gene): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.