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DNase I, recombinant, RNase-free, GMP Grade

from bovine pancreas, expressed in Pichia pastoris, lyophilizate

For further processing only.

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Order information
DNase I, recombinant, RNase-free, GMP Grade material number and pack size:
Material Number Pack Size
03724751103 Minimum order size of 80 KU (Pack size: 4 KU)
Will be supplied as ''DNase I, rec., lyo., 4 KU''. Unit of measure is ''piece".
Recombinant DNase I is an essential tool for all applications requiring DNA-free RNA templates.
DNase I, recombinant, RNase-free, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a glycoprotein of a molecular weight of approximately 39 kD. DNase I, recombinant, RNase-free, is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
The enzyme is highly purified and rigorously tested for contaminating RNase and protease activity for superb RT-PCR.
  • Achieve reliable results with undegraded and stable RNA.
    Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high sensitivity of your transcription reaction.
Use DNase I, recombinant, for isolation of DNA-free RNA in diagnostic and therapeutic applications:
  • To ensure that RT-PCR templates are free of genomic DNA
  • To remove DNA templates after in vitro transcription of RNA
Nomenclature: DNase I
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA.
Appearance: White powder
Activity (with calf thymus DNA): ≥4.0 kU/vial
Ribonucleases (up to 15 U with MS II RNA /4 h/ 37°C): Not detectable
Manufactured acc. to GMP: Corresponds to specification
Filtered through 0.2 μm pore size Membrane, microbiologically tested: Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.