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T7 RNA Polymerase, recombinant, GMP Grade

expressed in E. coli

For further processing only.

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Order information
T7 RNA Polymerase, recombinant, GMP Grade material number and pack size:
Material Number Pack Size
08140669103 10 mL
Will be supplied as "T7 RNA Polymerase, rec., GMP Grade". Unit of measure is "piece".
T7 RNA Polymerase is used for in vitro transcription of RNA from a DNA template.
T7 RNA Polymerase exhibits high specificity for T7 promoter sequence and requires Mg2+ as a cofactor for the synthesis. The enzyme efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts.
Using fit-for-purpose raw materials enables you to focus on drug design and clinical studies, and increase certainty in your manufacturing process.

Roche T7 RNA Polymerase is
  • manufactured in a state-of-the-art facility under GMP quality standards
  • animal-free and ß-lactam antibiotic-free
  • available in scale and quality for every stage — from development to manufacturing
  • backed by the expertise of a global pioneer in diagnostics and pharmaceuticals
It is the key enzyme for in vitro transcription of mRNA. It is designed fit-for-purpose to meet the manufacturing needs of therapeutic mRNA.
Molecular weight: 98 kD
Supplied as solution
Appearance: Clear, colorless solution
Volume Activity: ≥ 1000 kU/mL
Protein (abs 280nm): 0.8-1.2 mg/mL
Purity (SDS-PAGE) ≥ 95 %
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): not detectable
RNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Total Bioburden: ≤ 10 CFU/mL
E.coli HC DNA ≤ 100 pg/mg protein: corresponds
E.coli HC protein ≤ 50 ppm: corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months.