T7 RNA Polymerase, recombinant, GMP Grade
expressed in E. coli
For further processing only.
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T7 RNA Polymerase, recombinant, GMP Grade material number and pack size: Material Number Pack Size 08140669103 10 mL
- T7 RNA Polymerase is used for in vitro transcription of RNA from a DNA Template.
- T7 RNA Polymerase is a key enzyme for RNA in vitro transcription reactions to produce e.g. therapeutic mRNA.
Appearance: Clear, colorless solution
Volume Activity: ≥ 1000 kU/mL
Protein (abs 280nm): 0.8-1.2 mg/mL
Purity (SDS-PAGE) ≥ 95 %
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): not detectable
RNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Total Bioburden: ≤ 10 CFU/mL
E.coli HC DNA ≤ 100 pg/mg protein: corresponds
E.coli HC protein ≤ 50 ppm: corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months.