For further processing into IVD products and medical devices only. Others Terminal Transferase recombinant Terminal Transferase, recombinant 3.6.14.3.7.2 from calf thymus, expressed in E. coli, solution 03 289 869 103 3 289 869 103 03289869103 3289869103 03289869103 Terminale Transferase, recombi Terminal Transferase, recombinant 07613336133156 Reagents, kits custom fill Not Available Custom Biotech product. Please contact your local representative. Will be supplied as "Terminale Transferase, recombi". Unit of measure is "kU". 9027-67-2 en Terminal Transferase catalyzes the template independent addition of deoxy- and dideoxynucleoside triphosphates to the 3'-OH ends of double- and single-stranded DNA fragments and oligonucleotides. Terminal Transferase incorporates digoxigenin-, biotin-, and fluorochrome-labeled deoxy- and dideoxynucleoside triphosphates, as well as radioactively labeled deoxy- and dideoxynucleoside triphosphates. The supplied 5x concentrated reaction buffer facilitates optimal tailing of all types of double-stranded DNA ends: blunt ended, with 3' overhang, or with 5' overhang. en Terminal Transferase catalyzes the addition of deoxy- and dideoxynucleoside triphosphates to the 3'-OH ends of double- and single-stranded DNA fragments and oligonucleotides. en Use Terminal Transferase to add homopolymer tails to DNA fragments in cloning experiments, such as addition of overhanging ends onto cDNAs for easier cloning and labeling of 3' ends of double- and single-stranded DNA (e.g., oligonucleotides) with radioactive labeled nucleotides or nucleotides labeled with haptens, e.g., digoxigenin or biotin. en Appearance: Clear, colorless solutionStorage buffer: Potassium phosphate, 60 mmol/L; KCl, 150 mmol/L; 2-mercaptoethanol, 1 mmol/L; Tween 20, 0.1%; glycerol, 50% (v/v); pH approximately 7.2 at +4°CVolume activity (Co2+): ≥400x103 U/mLSpecific activity (Co2+): ≥200x103 U/mgUnit definition: One unit is the enzyme activity that leads to an incorporation of 1 nmol dTMP into acid insoluble products within 30 minutes at +37°C under assay conditions (cacodylate, 200 mmol/L; Co2+, 1 mmol/L) using d(pT)6 as primer.Unspecific endonucleases (MWM II DNA): Not detectable in up to 400 U after 4 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 400 U after 4 hours incubation at +37°C.Function test (tailing reaction on a 30-mer oligonucleotide): Corresponds to specificationStability: At -15 to -25°C within specification range for 24 months. en

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Terminal Transferase, recombinant

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