Others Transcriptor Reverse Transcriptase recombinant expressed in E. coli Transcriptor Reverse Transcriptase For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only acc. to Art. 56 (3) and 3 no. 23 REACH Regulation. 3.6.14.3.13.1 recombinant, expressed in E. coli 03531252103 Transcriptor Bulk Transcriptor Reverse Transcriptase 07613336133248 Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Transcriptor Bulk". Unit of measure is "kU". The enzyme is supplied without reaction buffer. 9068-38-6 en Use Transcriptor Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for: Two-step RT-PCR applications using conventional thermal cyclers or real-time PCR instrumentsRT-PCR for detection of viral RNATMA and NASBA nucleic acid amplification methodsSynthesis of full-length cDNA up to 14 kb for libraries or cloningRapid amplification of cDNA end (RACE) en Transcriptor Reverse Transcriptase is the robust recombinant reverse transcriptase with thermostability up to +60°C, for transcription of RNA fragments up to 14 kb in two-step RT-PCR applications. en Appearance: Clear, colorless solutionStorage buffer: Potassium phosphate, 200 mmol/L; DTT, 2 mmol/L; Triton X-100, 0.2% (v/v); glycerol, 50% (v/v), pH approximately 7.2Volume activity: ≥20 U/μLSpecific activity: ≥50 kU/mg proteinUnit definition: One unit Transcriptor Reverse Transcriptase is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.Purity (SDS PAGE): ≥90%Unspecific endonucleases (MWM III DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 40 U after 4 hours incubation at +37°C.Function test in RT-PCR (human skeletal muscle total RNA, 10 kb dystrophin gene fragment): Corresponds to referenceFunction test in real-time RT-qPCR using the LightCycler® instrument (PBGD gene fragment from RNA standards): Corresponds to referenceAnimal-derived additives: NoneStability: At -15 to -25°C within specification range for 12 months. en