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Transcriptor Reverse Transcriptase

recombinant, expressed in E. coli

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
Transcriptor Reverse Transcriptase material number and pack size:
Material Number Pack Size
03531252103 custom fill
Will be supplied as "Transcriptor Bulk". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
Transcriptor Reverse Transcriptase is the robust recombinant reverse transcriptase with thermostability up to +60°C, for transcription of RNA fragments up to 14 kb in two-step RT-PCR applications.
Use Transcriptor Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:
  • Two-step RT-PCR applications using conventional thermal cyclers or real-time PCR instruments
  • RT-PCR for detection of viral RNA
  • TMA and NASBA nucleic acid amplification methods
  • Synthesis of full-length cDNA up to 14 kb for libraries or cloning
  • Rapid amplification of cDNA end (RACE)
Transcriptor Reverse Transcriptase offers higher thermostability compared to the native forms of AMV or M-MLV reverse transcriptase, allowing higher temperatures for reverse transcription, achieving high performance with GC-rich RNA fragments and difficult secondary structures.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, unwinding activity, RNase H (degrading RNA in RNA:DNA hybrids)
Recommended reaction temperature: +42 to +65°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg2+
Appearance: Clear, colorless solution
Storage buffer: Potassium phosphate, 200 mmol/L; DTT, 2 mmol/L; Triton X-100, 0.2% (v/v); glycerol, 50% (v/v), pH approximately 7.2
Volume activity: ≥20 U/μL
Specific activity: ≥50 kU/mg protein
Unit definition: One unit Transcriptor Reverse Transcriptase is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 U after 4 hours incubation at +37°C.
Function test in RT-PCR (human skeletal muscle total RNA, 10 kb dystrophin gene fragment): Corresponds to reference
Function test in real-time RT-qPCR using the LightCycler® instrument (PBGD gene fragment from RNA standards): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.