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NxtScript Reverse Transcriptase, concentrate

mutant from Moloney Murine Leukemia Virus, expressed in E. coli

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
NxtScript Reverse Transcriptase, concentrate material number and pack size:
Material Number Pack Size
07051166103 custom fill
Will be supplied as "NxtScript RT, conc.". Unit of measure is "MU".
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Overview
M-MLV Reverse Transcriptase mutant designed for high thermostability and comparable performance to the market leader.
  • Reverse transcribe difficult templates.
    The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).
  • Achieve higher yield.
    NxtScript RT lacks RNase H activity. This results in higher cDNA yields.
  • Stay specific.
    Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target.
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Application
Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:
  • Two-step RT-PCR applications
  • RT-PCR for detection of viral targets
  • RT-PCR for detection of mRNA targets, such as cancer biomarkers
  • TMA and NASBA nucleic acid amplification methods
  • Generation of full-length cDNA libraries
  • Rapid amplification of cDNA ends (RACE)
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Specification
NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg2+
Appearance: Clear, colorless solution
Activity: ≥250 U/μL
Unit definition: One unit NxtScript Reverse Transcriptase is defined as the amount of enzyme which incorporates 1nmol [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.
Purity (HPLC): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.
Stability: At -15 to -25°C wihtin specification range for 12 months.