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T4 DNA Ligase

recombinant form of the enzyme from T4 phage, solution

For further processing only.

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Order information
T4 DNA Ligase material number and pack size:
Material Number Pack Size
10909246103 custom fill
Will be supplied as "DNA Ligase, T4 from T4-infected EcoNM989". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
Use T4 DNA Ligase for ligation of DNA fragments.
Use T4 DNA Ligase to ligate DNA fragments with blunt or overlapping ends.
Enzyme activities: T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighbouring 3'-hydroxyl and 5'-phosphate ends in double-stranded DNA. Sticky- and blunt-ended DNA fragments are ligated. Single-stranded nicks in double-stranded DNA are also closed.
Appropriate ligation buffer, 10x concentrated: Tris/HCl, 660 mmol/L; MgCl2, 50 mmol/L; DTT, 50 mmol/L; ATP, 10 mmol/L, pH 7.5 at +20°C (Note: ATP is not stable).
pH optimum: 7.2-7.8
Divalent ion requirement: Mg2+
Inactivation: After 10 minutes heat denaturation at +65°C ligase activity is stopped.
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 60 mmol/L; DTE, 5 mmol/L; EDTA, 1 mmol/L; glycerol, 50% (v/v); pH approximately 7.5 at +4°C
Volume activity: ≥5 U/μL
Unit definition: One unit T4 DNA Ligase is defined as the amount of enzyme which converts 1 nmol of [32P] from pyrophosphate into Norit-absorbable material in 20 minutes at +37°C.
Glycosylases (M13mp11(U) ssDNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 15 U after 4 hours incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.