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Biotin-16-ddUTP

1 mM solution

For further processing only.

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Order information
Biotin-16-ddUTP material number and pack size:
Material Number Pack Size
11431692103 custom fill
Will be supplied as ''Biotin-16-ddUTP''. Unit of measure is ''nmol''.
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Overview
Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates.
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Application
Biotin-16-ddUTP is used as a substrate for:
  • Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • T4 DNA polymerase
  • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor)
Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant.
The biotin-labeled oligonucleotide can be used as a hybridization probe for:
  • DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3' end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine-triphosphate (biotin-ddUTP).
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Specification
Nomenclature: Biotin-16-2',3'-dideoxy-uridine-5'-triphosphate
Formula: C32H48N7O17P3SLi4
Molecular weight: 955.5 D
Appearance: Clear, colorless solution
Biotin-16-ddUTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test using DIG Oligonucleotide 3'-End-Labeling Kit, 2nd generation (DIG is replaced by Biotin-16-ddUTP): Corresponds to reference
Stability: At -15 to -25°C within specification range for 24 months.