NxtScript DNA Master
For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.
NxtScript DNA Master material number and pack size: Material Number Pack Size 07368143103 5 mL 5 x conc.
Reversible hot start DNA master mix for multiplexing and as DNA master mix component in RT-PCR.
NxtScript DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for multiplex qPCR or qRT-PCR. It uses aptamer-mediated reversible hot start technology for specific priming and fast PCR. The mix contains dNTP mix with dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.
- Save time.
Take advantage of the aptamer technology and run a fast PCR protocol.
- Achieve high sensitivity.
Detect low copy numbers of DNA or RNA targets with higher sensitivity using a 5x master mix concentration.
- Ready for automation.
Set up your reaction at room temperature.
- Save time.
- Use NxtScript Reverse Transcriptase (07051166103) in combination with NxtScript DNA Master to run highly sensitive qRT-PCR reactions to detect RNA pathogens.
NxtScript DNA Master is a sensitive and robust reaction mix for detection of DNA and RNA pathogens. It is stable at +2 to +8°C for 3 months and in its final reaction setup, for 4 hours at room temperature.
Appearance: Clear colorless solution
Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.