ActiTaq exo Genotyping Master
5x concentrated
For further processing only.
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Will be supplied as "ActiTaq delta exo Genotyping Master". Unit of measure is "mL".
ActiTaq exo Genotyping Master material number and pack size: Material Number Pack Size 06362281001 50 mL -
Hot start DNA master mix containing a 5'-3' exonuclease deficient hot start Taq DNA polymerase for optimal detection of mismatches.
The 5x concentrated master mix contains a chemically modified truncated Taq DNA Polymerase and all reagents (except primers, probes, and template) needed for real-time DNA detection assays with various probe formats except for hydrolysis probes due to the lack of 5'-3' exonuclease activity.
- Optimize your SNP analysis.
Discriminate paired and unpaired primer ends using an enzyme optimized for allele-specific PCR. - Simplify PCR set-up.
Pipett and handle the hot start reaction mix at ambient temperature. - Simplify assay design.
Create capable PCR assays with a minimum of optimization efforts, also multiplex PCR applications.
- Optimize your SNP analysis.
- Use ActiTaq exo DNA Polymerase for:
- SNP analysis and genotyping
- Allele-specific PCR
- Multiplexing up to 350 bp
- Random primed PCR
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The master mix can be stored in the refrigerator (+2 to +8°C) for at least 4 week without loss of activity and performance. The complete PCR mix (Master + primers + probes + template) is stable for up to 24 hours at +15 to +25°C.
Appearance: Clear colorless solution
Performance test in qPCR (amplification with ApoB fragment): Corresponds to specification
Sensitivity (≤100 copies; negative control ≥40 cycles): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.