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Collagenase P

from Clostridium histolyticum

For further processing only.

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Order information
Collagenase P material number and pack size:
Material Number Pack Size
11215809103 custom fill
Will be supplied as "Collagenase P from Clostridium histoly.". Unit of measure is "G".
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Overview
The product is prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.
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Application
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.
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Specification
Appearance: Light brown to brown lyophilized substance
Activity (+25°C; with PZ-Pro-Leu-Gly-Pro-D-Arg ): ≥1.5 U/mg,lyophilized material

Contaminants expressed as percentage of collagenase P activity:
Clostripain: no limit (U/mg lyophilized material)
Proteases (azocoll): no limit (U/mg lyophilized material)
Trypsin (with BAEE): no limit (U/mg lyophilized material)
Performance test with pancreatic cells:corresponds
Stability: At + 2°C to + 8°C: within specification range for 12 months; stored dry and protected from light.