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QC Sample Preparation Kit

For use in quality control / manufacturing process only.

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Order information
QC Sample Preparation Kit material number and pack size:
Material Number Pack Size
08146829001 1 kit
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Overview
Preparation of cell culture samples before PCR test detection of mycoplasma or residual DNA from CHO cells and from E. coli.
The kit is designed to be used with the MycoTOOL Mycoplasma Detection Amplification Kit. Additionally it also can be used for the Residual DNA CHO Kit and Residual DNA E. coli Kit.
Speed up by optimized extraction protocols to prepare samples for PCR tests and gain safety as the Kit does not include any Triton-X .
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Application
The QC Sample Preparation Kit offers optimized extraction protocols to prepare sample for further PCR tests.

Detection of mycoplasma contamination
  • Using a PCR block cycler, refer to the method sheet (instructions for use) of the MycoTOOL Mycoplasma Detection Amplification Kit.
    - for testing of CHO or SP2/0 cells with 5 x 106 cells/ml (standard protocol)
    - for testing of CHO or SP2/0 samples up to 1 x 108 cells/ml (protocol for high cell density)
  • Using a real-time PCR instrument, refer to the method sheet (instructions for use) of the MycoTOOL Real-Time PCR Kit for testing of mammalian cell culture samples. This PCR kit contains a control plasmid (Recovery Control) that can be added to the sample material to prevent false negative results.
Detection of Host Cell DNA
  • Preparation of samples for testing 100 samples (per 100 μL) from fermentation process for detection of residual DNA from E. coli or CHO cells.
  • Refer to the method sheet (instructions for use) of the Residual DNA E. coli Kit, or of the Residual DNA CHO Kit. These PCR kits contain Standard DNA that can be added to the sample material to prevent false negative results and a workflow negative control as well.
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Specification
It is designed for:
  • 13 extractions from mammalian cell culture samples (per 1 mL)
  • or 5 extractions from high density cell culture samples (per 1 mL)
  • or 100 extractions form fermentation process (per 100 μL).
Stability: At +15 to +25 °C within specification for 12 months.