4-Nitrophenyl Phosphate (pNPP)
disodium salt, crystalline powder
For further processing only.
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4-Nitrophenyl Phosphate (pNPP) material number and pack size: Material Number Pack Size 10004847103 custom fill
Additional formulation: Tablets are available on request.
- Use pNPP as a substrate for alkaline phosphatase.
Formula: C6H4NO6PNa2 x 6 H2O
Molecular weight: 371.1 D (pNPP: 219.1 D)
Detection: at 405 nm Appearance: White to slightly yellow crystalline powder
Solubility: Clear, colorless to slightly yellow solution in water (c=50 mg/mL)
pH value: 9±1
4-NPP-Na2 x 6 H2O (calculated from value found enzymatically): ≥95%
4-NPP (enzymatic): ≥56%
Na (flame photometric): 13±1%
Water (K. Fischer): 28±3%
4-Nitrophenol (free): ≤0.07%
Pi: ≤ 0.3%
Blank (with TC APopt. A/30 min) : ≤0.015
Reactions rates (AP): 100±5%
Stability: At +2 to +8°C within specification range for 24 months. Protect from light.