cOmplete His-Tag Purification Resin
pre-charged with Ni2+, ready-to-use
For further processing only.
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cOmplete His-Tag Purification Resin material number and pack size: Material Number Pack Size 08778850001 800 ml settled resin volume
The cOmplete His-Tag Purification Resin is an innovative solution for the purification of histidine-tagged proteins via batch or liquid chromatography procedures.
In contrast to all currently available resins, which are primarily based on NTA or IDA chelator chemistry, this resin is the only resin on the market which tolerates buffers that contain EDTA. Because EDTA is a known inhibitor of metalloproteases - which are frequently present in any cell type - this chromatography material provides the option to increase the protection of your target protein by supplementing your buffers with EDTA.
This resin therefore allows increased protection of your proteins against degradation and, at the same time, enriches the protein using the well-established affinity purification method.In addition, the resin maintains its binding capacity whether you are using low- or high-molecular weight proteins, and its specificity allows a one-step purification . By using one of the strongest chelators available, minimal contamination of your flow-through fractions with metal ions is ensured, safeguarding your downstream applications.
- cOmplete His-Tag Purification Resin can be used for small or large-scale purification of His-tagged proteins in biopharma manufacturing, yielding highly purified proteins from crude lysates. This resin can be used for batch or column chromatography procedures, and allows for target protein purification using both native and denaturing conditions. cOmplete His-Tag Purification Resin is compatible with common reducing agents and chelators while preventing contamination of the protein preparation with heavy metals.
cOmplete His-Tag Purification Resin is a sepharose-based, pre-charged, ready-to-use Ni2+ chelate resin for small and large-scale purification of His-tagged proteins. It allows for the production of highly pure proteins from crude lysates, using a one-step purification process.
Matrix: Sepharose-CL 6B
Bead size: 45 to 165 μM
Binding Capacity: ≥40 mg protein per mL
Note: The binding capacity of the resin to varíous types of proteins may vary according to the protein characteristics such as the size of the protein.