Peroxidase (POD), Grade II
from horse radish, lyophilizate
For further processing only.
expand | collapse
Peroxidase (POD), Grade II material number and pack size: Material Number Pack Size 11378783103 custom fill 11378783101 1 MU package
11378783101: Unit of measure for 1 MU-package is “piece”.
11378783103: Unit of measure for custom fill is "MU".
- Use Peroxidase (POD), Grade II, for the oxidation of reduced dyes in the indicator reaction of many diagnostic tests, e.g., for the determination of blood glucose, triglycerides or lactate. It may also be used as a marker enzyme for enzyme immunoassays (EIA).
Nomenclature: Donor:hydrogen-peroxide oxidoreductase
Molecular weight: 40 kD
Structure: Glycoprotein with one mole of protoheme IX
Isoelectric point: 7.2
a) Hydrogen acceptors:
H2O2 9 x 108 [L x mol-1 x s-1]
methyl peroxide 1.5 x 106 [L x mol-1 x s-1]
ethyl peroxide 3.6 x 106 [L x mol-1 x s-1]
b) Hydrogen donors: Many
Inhibitors: Cyanide, sulfide, fluoride, azide, hydroxylamine, hydroxyl ions
Activators: Peroxidation of o-dianisidine is accelerated by ammonia, pyridine, imidazole at pH values >7.0
pH optimum: 6.0-6.5 (see figure)
Temperature dependence: See figure
pH stability: 4.0-10.0 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: Peroxidase is specific for the hydrogen acceptor; only H2O2, methyl- and ethylperoxides are active. In contrast the enzyme is not specific for the hydrogen donor. A large number of phenols, aminophenols, diamines, indophenols, leucocyte dyes, ascorbate and several amino acids react. Appearance: Red-brown lyophilizate
Solubility: Clear, red-brown solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 6.6-7.6
Activity: ≥200 U/mg lyophilizate
Purity number (A403/A275): 2.0-3.5
A500 (100 U/mL): ≤0.120
Contaminants (expressed as percentage of Peroxidase activity):
Contaminating oxidases: ≤0.00005
Phosphatase, acidic: ≤0.001
Glucose: ≤0.25 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 24 months. Store dry.