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Urease

from jack bean, lyophilizate

For further processing only.

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Order information
Urease material number and pack size:
Material Number Pack Size
11759132103 custom fill
Will be supplied as "Urease, Lyo., SQ". Unit of measure is "MU".
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Overview
Hydrolase that catalyzes the breakdown of urea in carbon dioxide and ammonia.
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Application
Use Urease in diagnostic tests for the determination of urea in combination with Glutamate Dehydrogenase, Catalog No. 11 434 993 103.
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Specification
Nomenclature: Urea amidohydrolase
Molecular weight: 480 kD
Isoelectric point: 5.0-5.1
Michaelis constant (Phosphate buffer, pH 7.0; +25°C):
Urea: 1.05 x 10-2 mol/L
Inhibitors: Na+, K+, NH4+; suramin and thiourea are competitive inhibitors.
Activators: Pi
pH optimum: 7.5 (see figure)
Temperature dependence: See figure
pH stability: 6.0-9.5 (see figure)
Thermal stability: Up to +70° C (see figure)
Specificity: Urease is specific for urea.
Appearance: Almost white lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
pH value (c=20 mg/mL in water): 6.0-7.0
Activity (+25° C, urea): ≥45 U/mg lyophilizate
Specific activity: ≥600 U/mg protein
Protein (Biuret): ≤0.15 mg/mg lyophilizate
Contaminants (expressed as percentage of Urease activity):
L-Arginase: ≤0.002
NH4: ≤1.5 μg/KU
Stability: At +2 to +8°C within specification range for 12 months. Store dry.