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from jack bean, lyophilizate

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Urease material number and pack size:
Material Number Pack Size
11759132103 custom fill
Will be supplied as "Urease, Lyo., SQ". Unit of measure is "MU".
Hydrolase that catalyzes the breakdown of urea in carbon dioxide and ammonia.
Use Urease in diagnostic tests for the determination of urea in combination with Glutamate Dehydrogenase, Catalog No. 11 434 993 103.
Nomenclature: Urea amidohydrolase
Molecular weight: 480 kD
Isoelectric point: 5.0-5.1
Michaelis constant (Phosphate buffer, pH 7.0; +25°C):
Urea: 1.05 x 10-2 mol/L
Inhibitors: Na+, K+, NH4+; suramin and thiourea are competitive inhibitors.
Activators: Pi
pH optimum: 7.5 (see figure)
Temperature dependence: See figure
pH stability: 6.0-9.5 (see figure)
Thermal stability: Up to +70° C (see figure)
Specificity: Urease is specific for urea.
Appearance: Almost white lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
pH value (c=20 mg/mL in water): 6.0-7.0
Activity (+25° C, urea): ≥45 U/mg lyophilizate
Specific activity: ≥600 U/mg protein
Protein (Biuret): ≤0.15 mg/mg lyophilizate
Contaminants (expressed as percentage of Urease activity):
L-Arginase: ≤0.002
NH4: ≤1.5 μg/KU
Stability: At +2 to +8°C within specification range for 12 months. Store dry.