Lipoprotein Lipase, chemically modified
from Pseudomonas species, lyophilizate
For further processing only.
expand | collapse
Lipoprotein Lipase, chemically modified material number and pack size: Material Number Pack Size 11145991103 custom fill
- Enzyme that hydrolyzes triglycerides into three free fatty acids and glycerol.
- Use Lipoprotein lipase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase, Catalog Nos. 10 539 937 103 or 11 499 530 103 and Glycerol-3-phosphate Dehydrogenase,Catalog Nos. 11 654 730 103 or 11 582 003 103.
The chemical modification generates an increased liquid stability of the enzyme.
Nomenclature: Triacylglycero-protein acylhydrolase
Molecular weight: 47 kD
Effectors: Hg2+, Ag+, Cr2+, Sn2+, Cu2+ and ionic detergents inhibit. Mg2+, sodium cholate and BSA stabilize the enzyme. 4-Chloromercuribenzoate (2 mmol/L), monoiodoacetate (2 mmol/L), NaF (20 mmol/L), NaN3 (20 mmol/L), EDTA (5 mmol/L) and 2-phenanthroline (2 mmol/L) do not affect the enzyme activity while SDS (0.1% (w/v)) is inactivating.
pH optimum: 7.5 (see figure)
pH stability: 6.0-10.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Lipoprotein Lipase has both lipolytic and sterol ester hydrolytic activities. It hydrolyzes triacylglycerols in chylomicrons, lipoproteins and diacylglycerols. With human plasma as substrate triglycerides are hydrolyzed more rapidly than cholesterol esters. The effects of pH and ionic strength on the enzymatic activity are somewhat different between the hydrolysis of triglyceride and of cholesterol ester depending on the different states of these substrates in the plasma or the transfer of the reaction products at the interface of substrates.
Lipolytic activity (Substrate, Number of C-atoms to number of double bonds, Relative rate):
olive oil: 94%
triolein (18:1): 100%
tripalmitin (16:0): 2%
trimyristin (14:0): 7%
trilaurin (12:0): 4%
tricaprin (10:0): 17%
tricaprylin (8:0): 64%
tricaproin (6:0): 2%
tributyrin (4:0): 2%
tripropionin (3:0): 2%
triacetin (2:0): 1%
Remark: Chemically modified Lipoprotein Lipase (LPL) is more hydrophilic than native LPL. Carryover effect is therefore reduced. Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/mL)
Activity (+25°C, cholesterol oleate): ≥10 U/mg lyophilizate
Contaminants (expressed as percentage of Lipoprotein Lipase activity):
''NADH oxidase'': ≤0.001
Stability: At +2 to +8°C within specification range for 12 months. Store dry.