from Pseudomonas species, expressed in E.coli, lyophilizate
For further processing only.
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Creatininase material number and pack size: Material Number Pack Size 11865471103 custom fill
- Hydrolase for creatinine determination that catalyzes the conversion of creatinine to creatine.
- Use Creatininase in diagnostic tests for the determination of creatinine in combination with Creatinase, Catalog No. 11 799 142 103 and Sarcosine Oxidase, Catalog No. 11 378 856 103.
Nomenclature: Creatinine amidohydrolase
Molecular weight (gel filtration): 175 kD
Structure (SDS PAGE): 8 equal subunits (23 kD + zinc)
Isoelectric point (IEF): 4.7
Michaelis constants (Glycylglycine buffer, pH 8.0, +25°C):
Creatinine: 3 x 10-2 mol/L
Creatine: 6 x 10-2 mol/L
Inhibitors: Hg2+, Fe3+, Cu2+ (1 mmol/L), N-bromosuccinimide, o-phenanthronline, 4-chloromercuribenzoate. The enzyme is sensitive against photooxidation. A stable, inactive apoenzyme free of zinc can be obtained after EDTA incubation which can be reactivated completely with Zn2+, Mn2+, Mg2+, Co2+, Fe2+ or Ni2+ (1 mmol/L).
Activators: Mn2+, Mg2+. The enzyme requires metal ions. Phenylmethylsulfonylfluoride and iodoacetamide do not react.
pH optimum: 7.8 (see figure)
Temperature dependence: See figure
pH stability: 7.5-9.0 (see figure
Thermal stability: Up to +65°C (see figure)
Specificity: Creatininase is specific for creatinine. It also reacts with glycocyamidine and glycocyamine. It does not react with hydantoin and its derivatives. Appearance: White to slightly yellowish lyophilizate
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 7.0-8.0
Activity (+25°C, creatinine): ≥55 U/mg lyophilizate
Specific activity: ≥250 U/mg protein
Protein (Biuret): 0.10-0.35 mg/mg lyophilizate
Contaminants (expressed as percentage of Creatininase activity):
Contaminating oxidases (FOX): ≤0.001
Creatinine deaminase: ≤0.0015
Kinase test: ≤0.01
Proteases (casein/resorufin, 2 hours stress duration): ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Store dry. Protect from light.