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from Pseudomonas species, expressed in E.coli, lyophilizate

For further processing only.

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Creatininase material number and pack size:
Material Number Pack Size
11865471103 custom fill
Will be supplied as "Creatininase, Recombinant Lyo". Unit of measure is "MU".
Hydrolase for creatinine determination that catalyzes the conversion of creatinine to creatine.
Use Creatininase in diagnostic tests for the determination of creatinine in combination with Creatinase, Catalog No. 11 799 142 103 and Sarcosine Oxidase, Catalog No. 11 378 856 103.
Nomenclature: Creatinine amidohydrolase
Molecular weight (gel filtration): 175 kD
Structure (SDS PAGE): 8 equal subunits (23 kD + zinc)
Isoelectric point (IEF): 4.7
Michaelis constants (Glycylglycine buffer, pH 8.0, +25°C):
Creatinine: 3 x 10-2 mol/L
Creatine: 6 x 10-2 mol/L
Inhibitors: Hg2+, Fe3+, Cu2+ (1 mmol/L), N-bromosuccinimide, o-phenanthronline, 4-chloromercuribenzoate. The enzyme is sensitive against photooxidation. A stable, inactive apoenzyme free of zinc can be obtained after EDTA incubation which can be reactivated completely with Zn2+, Mn2+, Mg2+, Co2+, Fe2+ or Ni2+ (1 mmol/L).
Activators: Mn2+, Mg2+. The enzyme requires metal ions. Phenylmethylsulfonylfluoride and iodoacetamide do not react.
pH optimum: 7.8 (see figure)
Temperature dependence: See figure
pH stability: 7.5-9.0 (see figure
Thermal stability: Up to +65°C (see figure)
Specificity: Creatininase is specific for creatinine. It also reacts with glycocyamidine and glycocyamine. It does not react with hydantoin and its derivatives.
Appearance: White to slightly yellowish lyophilizate
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 7.0-8.0
Activity (+25°C, creatinine): ≥55 U/mg lyophilizate
Specific activity: ≥250 U/mg protein
Protein (Biuret): 0.10-0.35 mg/mg lyophilizate
Contaminants (expressed as percentage of Creatininase activity):
ATPase: ≤0.01
Catalase: ≤2.0
Contaminating oxidases (FOX): ≤0.001
Creatinine deaminase: ≤0.0015
Kinase test: ≤0.01
Proteases (casein/resorufin, 2 hours stress duration): ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Store dry. Protect from light.