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Amino Acid Arylamidase

from Hog Kidney

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Order information
Amino Acid Arylamidase material number and pack size:
Material Number Pack Size
10150754103 custom fill
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Overview
Amino acid arylamidase (Aminopeptidase M) from hog kidney; a 280,000 MW metalloprotease which hydrolyzes proteins or peptides containing free alpha-amino or alpha-imino groups and those containing only L-amino acids.
Acetylation of the N-terminus blocks amino acid release, and proline is released slowly, especially where it occurs next to certain hydrophobic residues.
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Specification
Optimum pH: 7.0-7.5
Optimum temperature: +37°C
Appearance: White suspension in ammonium sulphate, 3.2 mol/L; MgCl2, 10 mmol/l; Tris, 10 mmol/l
pH value: 7.0 to 8.0
Specific activity (+25°C; leucine-4-nitranilide as substrate): 4 U/mg protein
Protein (biuret): no limit
Ammonium sulphate: 3.2 ± 0.2 mol/L
Contaminating oxidases (FOX): ≤3 %
SVD-free:corresponds
pH 5.5-treatment (30 min):corresponds
Stability: At +2°C to + 8°C: within specification range for 24 months.