from Lysobacter enzymogenes, lyophilizate
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Endoproteinase Lys-C material number and pack size: Material Number Pack Size 10476978103 3 U
- Endoproteinase Lys-C can be used for specific cleavage of peptides. Endoproteinase Lys-C is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a lyophilizate.
- Use Endoproteinase Lys-C for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.
Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin Appearance: White lyophilizate
Activity: ≥3 U/bottle
Specific activity: ≥150 U/mg protein
Unspecific cleavage peptides (HPLC, after 6 hours incubation): ≤5 area%
Stability: At +2 to +8°C within specification range for 12 months.