Alcohol Dehydrogenase
from yeast, lyophilizate
For further processing only.
| collapse
-
Will be supplied as "Alcohol Dehydrogenase, Yeast". Unit of measure is "MU".
Alcohol Dehydrogenase material number and pack size: Material Number Pack Size 11452541103 custom fill - Dehydrogenase that catalyzes the interconversion of alcohols to the corresponding aldehydes.
- Use Alcohol Dehydrogenase in diagnostic tests for the determination of alcohol or aldehyde (formate).
-
Nomenclature: Alcohol:NAD+ oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10-2 mol/L
NAD: 7.4 x 10-5 mol/L
Acetaldehyde: 7.8 x 10-4 mol/L
NADH: 1.1 x 10-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10-2 mol/L
NAD: 6.1 x 10-4 mol/L
Acetaldehyde: 6.7 x 10-4 mol/L
NADH: 1.8 x 10-5 mol/L
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg2+, Ag+ and Cu2+.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H2O2 and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure)
Temperature dependence: See figure
pH stability: 6.0-8.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.
Remarks:Alcohol dehydrogenase tends to show turbidity in solution at +37°C storage. Modified Alcohol dehydrogenase shows no turbidity for at least 4 weeks in solution at +37°C. Appearance: White lyophilizate (50 mg lyophilizate contain approximately 30 mg enzyme protein,15 mg sucrose, 5 mg phosphate)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, ethanol): No limit
Specific activity: ≥400 U/mg (protein)
Protein (Biuret): ≥0.5 mg/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry. -
