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Glycerol Kinase (GK)

from Bacillus stearothermophilus, lyophilizate

For further processing only.

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Glycerol Kinase (GK) material number and pack size:
Material Number Pack Size
11499530103 custom fill
Will be supplied as "GK, B.stearot., Lyo., w. Lactose". Unit of measure is "MU".
Enzyme that catalyzes the phosphorylation of glycerol to glycerol-3-phosphate.
Use Glycerol Kinase for diagnostic tests for the determination of triglycerides together with Glycerol-3-phosphate Oxidase, Catalog Nos. 11 582 003 103 and 11 654 730 103, and Lipoprotein Lipase, Catalog No. 11 145 991 103.
Nomenclature: ATP:glycerol 3-phosphotransferase
Molecular weight: 230 000 D (Sephadex G 200), 4 x 58 000 D (SDS-gel electrophoresis)
Michaelis constants (Glycine buffer, pH 9.8; +30°C):
Glycerol: 4.4 x 10-5 mol/L
Inhibitors: Unknown; Inhibitors of glycerokinase from Candida mycoderma do not inhibit the glycerokinase from Bacillus stearothermophilus.
pH optimum: 10.0-10.5 (see figure)
Temperature dependence: See figure
pH stability: 5.0-11.0 (see figure)
Thermal stability: Up to +60° C (see figure)
Appearance: White to slightly yellowish lyophilizate
Solubility: Clear, colorless solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 5.0-7.0
Activity (+25°C, glycerol): 18-25 U/mg lyophilizate
Specific activity: ≥80 U/mg protein
Protein (Biuret): 0.18-0.26 mg/mg lyophilizate
Contaminants (expressed as percentage of Glycerol Kinase activity):
ATPase: ≤0.005
Hexokinase: ≤0.01
"NADH oxidase": ≤0.005
Glycerol (enzymatic): ≤40 μg/kU
Stability: At +2 to +8°C within specification range for 12 months. Store dry.