Ascorbate Oxidase, chemically modified
from Cucurbita species, lyophilizate
For further processing only.
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Ascorbate Oxidase, chemically modified material number and pack size: Material Number Pack Size 11558668103 custom fill
- Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate.
- Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate or creatinine.
Nomenclature: L-ascorbate:oxygen oxidoreductase
Molecular weight: Approximately 140 kD
Isoelectric point: 5.0-6.0
Michaelis constant (Phosphate buffer, pH 5.6, +25°C):
L-ascorbate: 3 x 10-4 mol/L
Inhibitors: 4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
pH optimum: 5.6-7.0 (see figure for product Cat. No. 10 199 605 103)
Temperature dependence: See figure
pH stability: 6.5-9.0 (see figure for product Cat. No. 10 199 605 103)
Thermal stability: Up to +70°C (see figure for product Cat. No. 10 199 605 103)
Stability of the lyophilizate: Stable at +35°C for at least 3 weeks (see figure for product Cat. No. 10 199 605 103).
Stability in solution: See figure for product Cat. No. 10 199 605
Specificity: Several analogs of ascorbate react.
Remark: The modified enzyme is especially suited for liquid stable applications with extended shelf life requirements. Appearance: Turquoise lyophilizate
Solubility: Clear, slightly turquoise solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.2-8.2
Activity (+25°C, L-ascorbate): ≥120 U/mg lyophilizate
Specific activity (+25°C): ≥1,200 U/mg protein
Activity (+37°C, L-ascorbate): ≥180 U/mg lyophilizate
Specific activity (+37°C): ≥1,800 U/mg protein
Protein (BCA): 0.04-0.10 mg/mg lyophilizate
Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):
Glutamate oxalacetate transaminase (AST): ≤0.0003
Glutamate pyruvate transaminase (ALT): ≤0.0005
Contaminating oxidases (FOX): ≤0.0002
Stability: At -15 to -25°C within specification range for 12 months. Keep tightly sealed.