For use in quality control/manufacturing process only. Others FcRn Affinity Column Gen2 FcRn Affinity Column Gen2 PID00000006 prepacked column for affinity chromatography 09430857001 FcRn Affinity Column Gen2 FcRn Affinity Column Gen2 07613336220832 Reagents, kits 1 column Not Available CustomBiotech product. Please contact your local representative. Unit of measure is "piece". The FcRn Affinity Column Gen2 enables fast and convenient analysis of monoclonal IgG antibodies with regard to their structural integrity and for prediction of their half-life in vivo as a therapeutic. en The prepacked column containing the FcRn matrix is ready for use on common liquid chromatography systems for the chromatographic differentiation of structural variants of IgG. en FcRn affinity chromatography provides information about pharmacokinetical characteristics of IgG variants in an easy and standardized way, replacing laborious methods like surface plasmon resonance spectroscopy or manual ELISA testing (reference: T. Schlothauer et. al., mAbs 5:4, 576-586, 2013). en Prepacked chromatography column supplied with standard fittings for liquid chromatography systems (FPLC, HPLC).Matrix: FcRn protein bound to sepharose matrixVolume: Approximately 1 ml resin bed volumeBinding capacity: ≥ 100 µg IgGReusability: Stable for ≥ 100 injections if used as recommended in the instructionsMaximal system pressure: 20 bar (290 psi)Maximal flow rate: 0.5 mL/min en The column is designed for chromatographic differentiation of structural variants of monoclonal IgG. FcRn analysis can provide insights into the structural and functional integrity of therapeutic immunoglobulins and enables improved conclusions on the half-life of the immunoglobulins in vivo:The neonatal Fc receptor (FcRn) immobilized in this chromatography column has a specific binding affinity to IgG molecules. The strength of the binding depends on structural variations of the IgG, like different Fab fragments, mutations in the Fc part, antibody isotypes, oxidized forms, aggregates, immune complexes, or antibody-drug conjugates. The FcRn affinity chromatography enables differentiation of such variants by peak patterns and retention times in the elution profile.Furthermore, the FcRn receptor has a binding affinity also to serum albumin, and the FcRn affinity chromatography can also be used to characterize albumin preparations, for example, for differentiation of oxidized, aggregated, or conjugated variants.Proteins binding to FcRn:Human IgG1, IgG2, IgG3, IgG4Some IgG of non-human species are able to bind human FcRnHuman albumin (often used as a fusion protein for therapeutic proteins) en