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FcRn Affinity Column

Prepacked column with immobilized human FcRn receptor for affinity chromatography

For quality control/manufacturing of IVD/medical devices/pharmaceutical products only.

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Order information
FcRn Affinity Column material number and pack size:
Material Number Pack Size
08128057001 1 column
Unit of measure is "piece".
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Overview
The FcRn Affinity column can be used for a functional characterization of IgG and albumin, and for detection of variants thereof.
Biotinylated recombinant human FcRn is immobilized to a Streptavidin Sepharose matrix and packed into an FPLC column ready for use.
Laborious and time-consuming conventional methods can be replaced.

Instead use FcRn affinity columns for:
  • Superior results
  • Shorter time-to-results
  • Reduced hands-on time
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Application
The column is designed for chromatographic differentiation of structural variants of monoclonal IgG. FcRn analysis can provide insights into the structural and functional integrity of therapeutic immunoglobulins and enables improved conclusions on the half-life of the immunoglobulins in vivo:

The neonatal Fc receptor (FcRn) immobilized in this chromatography column has a specific binding affinity to IgG molecules. The strength of the binding depends on structural variations of the IgG, like different Fab fragments, mutations in the Fc part, antibody isotypes, oxidized forms, aggregates, immune complexes, or antibody-drug conjugates. The FcRn affinity chromatography enables differentiation of such variants by peak patterns and retention times in the elution profile.
Furthermore, the FcRn receptor has a binding affinity also to serum albumin, and the FcRn affinity chromatography can also be used to characterize albumin preparations, e.g. for differentiation of oxidized, aggregated or conjugated variants.

Proteins binding to FcRn:
  • Human IgG1, IgG2, IgG3, IgG4
  • Some IgG of non-human species are able to bind human FcRn
  • Human albumin (often used as a fusion protein for therapeutic proteins)
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Specification
Matrix: Streptavidin Sepharose
Bead size: 34 μm
Prepacked chromatography column with approximately 1 mL resin (resin bed volume) supplied with standard fittings for liquid chromatography systems (FPLC, HPLC)
Binding capacity: ≥ 100 µg IgG per mL bed volume of resin
Maximal system pressure and temperature: 20 bar at +25°C.
Maximal recommended volumetric flow rate: 0.5 mL/minute
HPLC: Corresponds to specification
Reusability: Columns can be reused for at least 100 injections.
Recommended buffer for long-term storage >4 weeks: 80% Buffer A and 20% Buffer B, pH 6.0, with addition of cOmplete ULTRA (1 tablet/50 mL) and ProClin 300 (0.05%) for stabilization.
Recommended running buffer during chromatography: 80 to 0% Buffer A, 20 to 100% Buffer B, pH 5.5 to 8.8.