For further processing into medical devices, IVD and pharmaceutical products only. Others Vaccinia mRNA Methyltransferase rec GMP Grade AOF Vaccinia mRNA Methyltransferase, rec., GMP Grade, AOF 102 1584029687338397 Warranty LimitationIt is the sole responsibility of the customer to determine the suitability of all raw materials, products and components in order to ensure that the final end-use product is safe for its end use; performs or functions as intended; and complies with all applicable legal and regulatory requirements. Roche makes no representations or warranties of any kind regarding the Product, express or implied, other than those expressly set forth in the terms and conditions of sale (if any), and disclaims all implied or statutory warranties including warranty of merchantability and fitness for a particular purpose. PID00001039 from vaccinia virus, expressed in E. coli, solution 10 267 500 101 10267500101 2'-O-Methyltransferase GMP Grade AOF 5mL 2'-O-Methyltransferase GMP Grade AOF 5mL Reagents, kits 5 mL Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "2'-O-Methyltransferase, GMP Grade, AOF". Unit of measure is "Piece". 10 267 500 103 10267500103 2'-O-Methyltransferase, GMP Grade, AOF 2'-O-Methyltransferase, GMP Grade, AOF Reagents, kits 70 mL Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "2'-O-Methyltransferase, GMP Grade, AOF". Unit of measure is "Piece". 2.1.1.57 en 2'-O-Methyltransferase (MetT) can be used for the enzymatic methylation of a 7-methylguanylate cap (Cap-0) structure, generating the Cap-1 structure. The Cap-1 structure is formed from m7G-ppp-RNA (RNA with Cap-0 structure, m7G-ppp-RNA, from the action of the Guanylyltransferase) by catalyzing a 2′-O-methylation in the ribose of the first transcribed nucleotide, consuming S-Adenosylmethionine (SAM) in the process. 5′-cap structures of mRNA are key to transcript stability, supporting translation and function, and reducing immunogenicity and degradation to achieve stable protein expression. en Appearance: clear colourless solution Volume activity: ≥ 45 U/ml Protein (OD 280 nm): 0.8 - 1.2 mg/ml Purity (HPLC): ≥90 % area% Unspecific Endoucleases: not detectable (up to 1 μl using MWM III-DNA / 1h / 37°C) Nicking activity: not detectable (up to 1 μl using pBR 322 DNA / 1h / 37°C) RNase activity: not detectable (up to 2 μl using MS 2-RNA / 1h / 37°C) Proteases: not detectable (50 μl / 15 min / 37°C) Bioburden: < 5 CFU/ml Endotoxins: ≤ 5 EU/mg Host cell DNA (≤ 100 pg/mg protein): corresponds Host cell protein (≤ 50 ppm): corresponds Identity: confirmed Heavy metals (Ni, Co, V): ≤10 ppm: corresponds Stability: At -15°C to -25°C within specification range for 24 months. en Vaccinia mRNA Methyltransferase, GMP Grade, AOF is supplied as a standalone component. en GMP Grade and fit-for-purpose vaccinia capping enzyme for in vitro mRNA synthesis applications. en

mRNA Vaccinia Capping System: Proven. Scalable.

Effective 5' capping is a critical step for ensuring the stability and translational efficiency of in vitro transcribed mRNA. Fit-for-purpose to manufacture mRNA for therapeutics and vaccines, the Roche CustomBiotech mRNA Vaccinia Capping System, featuring our new mRNA Guanylyltransferase and mRNA Methyltransferase (rec., GMP Grade, AOF), provides the performance and quality you need.

To reduce your overall process risk and simplify the path to regulatory approval, our manufacturing process is meticulously designed—from the master cell bank to final QC-release—to exclude or minimize potential contaminants.

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mRNA Vaccinia Capping System: Proven. Scalable.

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