{ "ProductData": { "ID": "3.1.5.1.1.2", "ProductType": "Others", "BrandName": "Urease", "ProductNameAddition": "from jack bean, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Urease" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Hydrolases", "NodeID": "70-023-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11759132103", "MaterialDescription": "Urease,Lyo.,SQ", "RegisteredProductName": "Urease", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Urease, Lyo., SQ\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Urease in diagnostic tests for the determination of urea in combination with Glutamate Dehydrogenase.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"Urease, Lyo., SQ\". Unit of measure is \"MU\".", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Hydrolase that catalyzes the breakdown of urea in carbon dioxide and ammonia.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Almost white lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
pH value (c=20 mg/mL in water): 6.0-7.0
Activity (+25° C, urea): ≥45 U/mg lyophilizate
Specific activity: ≥600 U/mg protein
Protein (Biuret): ≤0.15 mg/mg lyophilizate
Contaminants (expressed as percentage of Urease activity):
L-Arginase: ≤0.002
NH4: ≤1.5 μg/KU
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: Urea amidohydrolase
CAS Number: 9002-13-5
Molecular weight: 480 kD
Isoelectric point: 5.0-5.1
Michaelis constant (Phosphate buffer, pH 7.0; +25°C):
Urea: 1.05 x 10-2 mol/L
Inhibitors: Na+, K+, NH4+; suramin and thiourea are competitive inhibitors.
Activators: Pi
pH optimum: 7.5 (see figure)
Temperature dependence: See figure
pH stability: 6.0-9.5 (see figure)
Thermal stability: Up to +70° C (see figure)
Specificity: Urease is specific for urea.", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }

Urease

from jack bean, lyophilizate

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